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AB83135

Biotin Anti-RANTES antibody

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(1 Publication)

Rabbit Polyclonal RANTES antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Mouse samples. Cited in 1 publication.

View Alternative Names

Scya5, Ccl5, C-C motif chemokine 5, MuRantes, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES

2 Images
Sandwich ELISA - Biotin Anti-RANTES antibody (AB83135)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-RANTES antibody (AB83135)

To detect mouse RANTES by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83135 is required. This biotinylated polyclonal antibody, in conjunction with a appropriate anti-RANTES antibody as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse RANTES.

Western blot - Biotin Anti-RANTES antibody (AB83135)
  • WB

Supplier Data

Western blot - Biotin Anti-RANTES antibody (AB83135)

To detect murine RANTES by Western Blot analysis ab83135 can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Image shown is reducing conditions; Murine recombinant RANTES protein at 250 - 0.24 ng/ml.

Lane 1 is MW marker, lanes 2-12 murine recombinant RANTES protein.

All lanes:

Western blot - Biotin Anti-RANTES antibody (ab83135)

Predicted band size: 10 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

Yes

Applications

WB, sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>The detection limit for recombinant murine RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.25-1 µg/mL", "sELISA-species-notes": "<p>Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine RANTES.</p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification technique
Affinity purification Protein A
Purification notes
ab83135 is sterile filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
See full target information Ccl5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 32:403-412 PubMed36457234

2022

CCL6 promotes M2 polarization and inhibits macrophage autophagy by activating PI3-kinase/Akt signalling pathway during skin wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Feng,Yu Ji,Ce Zhang,Tingting Jin,Jingyu Li,Jincai Guo
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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