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AB271225

Biotin Anti-RELM beta antibody

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(3 Publications)

Rabbit Polyclonal RELM beta antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Retnlb aa 1 to C-terminus.

View Alternative Names

Fizz2, Retnlb, Resistin-like beta, Cysteine-rich secreted protein A12-beta, Cysteine-rich secreted protein FIZZ2, RELMbeta

2 Images
Sandwich ELISA - Biotin Anti-RELM beta antibody (AB271225)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-RELM beta antibody (AB271225)

Mouse RELMβ was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271225.

This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse RELMβ protein.

Western blot - Biotin Anti-RELM beta antibody (AB271225)
  • WB

Supplier Data

Western blot - Biotin Anti-RELM beta antibody (AB271225)

To detect mouse RELM beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.

When used in conjunction with compatible secondary reagents, the detection limit for recombinant mouse RELM beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane 1 : Marker

Lanes 2-12 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse RELM beta, respectively.

Reducing conditions.

All lanes:

Western blot - Biotin Anti-RELM beta antibody (ab271225)

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

sELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Retnlb aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99P86

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in PBS, 0.1% BSA
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RELM beta also known as Resistin-Like Molecule Beta or FIZZ2 is a cysteine-rich secreted protein with an approximate mass of 19 kDa. This protein expresses mainly in tissues like the colon small intestine and adipose tissue. It belongs to the resistin-like molecule (RELM) family which is known for its similarities in amino acid sequence and structural features. RELM beta usually gets secreted by epithelial cells particularly in the intestinal region which suggests its active involvement in the gut environment.
Biological function summary

The RELM beta protein modulates immune responses and interacts with elements of the metabolic processes. It plays a role in bridging innate and adaptive immunity affecting inflammatory cascades through its cytokine-like activities. Although it is not part of a large complex RELM beta influences receptor signaling pathways and can affect macrophages and other immune cells. Its activity on energy homeostasis and glucose metabolism indicates key interactions in metabolic pathways influencing overall cellular functions.

Pathways

RELM beta prominently interacts with pathways involved in inflammation and metabolism. It participates in the NF-kB signaling pathway which is a central mediator in inflammation and immune responses. Also it connects to the adipokine signaling pathway where it works alongside other resistin family proteins. Through these pathways RELM beta coordinates with proteins like TNF-alpha and IL-6 in modulating inflammation and energy balance.

RELM beta relates to conditions such as inflammatory bowel disease (IBD) and type 2 diabetes. Its increased expression associates with elevated inflammatory states found in IBD and insights suggest a role in disease progression through interactions with proteins like IL-1β. In type 2 diabetes the regulation of glucose homeostasis implicates RELM beta as a participant in metabolic dysregulation. Changes in RELM beta expression can influence risk factors potentially in conjunction with other mediators like insulin.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable hormone.
See full target information Retnlb
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 8:792540 PubMed35096968

2022

Characterization of an Aging-Based Diagnostic Gene Signature and Molecular Subtypes With Diverse Immune Infiltrations in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhao,Fengfeng Lv,Ye Zheng,Liqiu Yan,Xufen Cao

Journal of clinical laboratory analysis 36:e24232 PubMed34995016

2022

A combined biomarker panel shows improved sensitivity and specificity for detection of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Mao,Yong Tang,Ming-Jing Deng,Chun-Tao Huang,Dong Lan,Wen-Zheng Nong,Li Li,Qi Wang

Head & face medicine 17:20 PubMed34158059

2021

Investigating resistin like beta (RETNLB) as a tumor promoter for oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Jin,Hui Miao,Yuan-Wen Nie,Yang-Yang Lin
View all publications
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Product promise

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