Rabbit Polyclonal RELM beta antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Retnlb aa 1 to C-terminus.
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- Frontiers in molecular biosciences 8:7925402022Characterization of an Aging-Based Diagnostic Gene Signature and Molecular Subtypes With Diverse Immune Infiltrations in Atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhao,Fengfeng Lv,Ye Zheng,Liqiu Yan,Xufen CaoPubMed 35096968
- Head & face medicine 17:202021Investigating resistin like beta (RETNLB) as a tumor promoter for oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Jin,Hui Miao,Yuan-Wen Nie,Yang-Yang LinPubMed 34158059
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Retnlb aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99P86
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Mouse | Predicted | Predicted |
| Recombinant fragment - Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info 0.10000-0.20000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Alternative names
Fizz2, Retnlb, Resistin-like beta, Cysteine-rich secreted protein A12-beta, Cysteine-rich secreted protein FIZZ2, RELMbeta
Recommended products
Rabbit Polyclonal RELM beta antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Retnlb aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Retnlb aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99P86
- Purification technique
- Affinity purification
- Reconstitution
- Reconstitute in PBS, 0.1% BSA
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RELM beta also known as Resistin-Like Molecule Beta or FIZZ2 is a cysteine-rich secreted protein with an approximate mass of 19 kDa. This protein expresses mainly in tissues like the colon small intestine and adipose tissue. It belongs to the resistin-like molecule (RELM) family which is known for its similarities in amino acid sequence and structural features. RELM beta usually gets secreted by epithelial cells particularly in the intestinal region which suggests its active involvement in the gut environment.
- Biological function summary
The RELM beta protein modulates immune responses and interacts with elements of the metabolic processes. It plays a role in bridging innate and adaptive immunity affecting inflammatory cascades through its cytokine-like activities. Although it is not part of a large complex RELM beta influences receptor signaling pathways and can affect macrophages and other immune cells. Its activity on energy homeostasis and glucose metabolism indicates key interactions in metabolic pathways influencing overall cellular functions.
- Pathways
RELM beta prominently interacts with pathways involved in inflammation and metabolism. It participates in the NF-kB signaling pathway which is a central mediator in inflammation and immune responses. Also it connects to the adipokine signaling pathway where it works alongside other resistin family proteins. Through these pathways RELM beta coordinates with proteins like TNF-alpha and IL-6 in modulating inflammation and energy balance.
- Associated diseases and disorders
RELM beta relates to conditions such as inflammatory bowel disease (IBD) and type 2 diabetes. Its increased expression associates with elevated inflammatory states found in IBD and insights suggest a role in disease progression through interactions with proteins like IL-1β. In type 2 diabetes the regulation of glucose homeostasis implicates RELM beta as a participant in metabolic dysregulation. Changes in RELM beta expression can influence risk factors potentially in conjunction with other mediators like insulin.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Biotin Anti-RELM beta antibody (ab271225)
To detect mouse RELM beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant mouse RELM beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse RELM beta, respectively.
Reducing conditions.
All lanes: Western blot - Biotin Anti-RELM beta antibody (ab271225)
Predicted band size: 12 kDa
Sandwich ELISA - Biotin Anti-RELM beta antibody (ab271225)
Mouse RELMβ was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271225.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse RELMβ protein.
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