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AB34771

Biotin Anti-RFP antibody

4

(5 Reviews)

|

(107 Publications)

Anti-RFP antibody - Biotin conjugated (ab34771) is a rabbit polyclonal antibody detecting RFP in Western Blot.

- Over 90 publications
- Trusted since 2006

View Alternative Names

Red fluorescent protein drFP583, DsRed

1 Images
Western blot - Biotin Anti-RFP antibody (AB34771)
  • WB

Supplier Data

Western blot - Biotin Anti-RFP antibody (AB34771)

All lanes:

Western blot - Biotin Anti-RFP antibody (ab34771) at 1/1000 dilution

Secondary

All lanes:

Peroxidase streptavidin secondary antibody at 1/40000 dilution

Predicted band size: 26 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Protein within Sea anenome Red fluorescent protein drFP583. The exact immunogen used to generate this antibody is proprietary information.

Q9U6Y8

Specificity

<p>Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. ELISA was used to confirm specificity at less than 0&#46;1% of target signal.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Biotin Anti-RFP antibody (ab34771) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB). Trusted by the scientific community
Anti-RFP Biotin (ab34771) was first used in a scientific publication in 2006 and has been cited over 90 times in peer-reviewed journals.

Reviewed by scientists
Anti-RFP Biotin (ab34771) has over 5 independent reviews from customers.
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Properties and storage information

Form
Lyophilized
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
See full target information Red fluorescent protein drFP583
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Publications (107)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 45: PubMed40957681

2025

Sensory Deficits in Mice with Lateral Spinal Cord Hemisection Mimic the Brown-Séquard Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Henwood,Junkui Shang,Qiang Li,John Moth,John Henwood,Yang Yi,Dustin Green,Ajay Pal,Joseph Sandoval,Wei Li,Tiffany Dunn,Alfredo Sandoval,Jiewen Zhang,Subo Yuan,Bo Chen

BMC neuroscience 26:44 PubMed40691765

2025

Anatomical and behavioral characterization of three hemiplegic animal models.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Liu,Lingling Xu,Gefei Cheng,Yang Yang,Likun Yang,Yuhai Wang

Aging cell 24:e14499 PubMed39932851

2025

Caloric Restriction and Telomere Preservation in TERT Knockout Adipocyte Progenitors Does Not Rescue Mice From Metabolic Dysfunction due to a TERT Function in Adipocyte Mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanguo Gao,Yongmei Yu,Kristin Eckel-Mahan,Mikhail G Kolonin

Nature biomedical engineering 8:1379-1395 PubMed39025942

2024

Profiling protein-protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Changju Chun,Ja Min Byun,Minkwon Cha,Hongwon Lee,Byungsan Choi,Hyunwoo Kim,Saem Hong,Yunseo Lee,Hayoung Park,Youngil Koh,Tae-Young Yoon

Journal of extracellular vesicles 13:e12478 PubMed39016173

2024

HIV-1 Nef is carried on the surface of extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Christophe Vanpouille,Beda Brichacek,Tatiana Pushkarsky,Larisa Dubrovsky,Wendy Fitzgerald,Nigora Mukhamedova,Sofia Garcia-Hernandez,Doreen Matthies,Anastas Popratiloff,Dmitri Sviridov,Leonid Margolis,Michael Bukrinsky

Cell 187:3427-3444.e21 PubMed38733990

2024

Spinal projecting neurons in rostral ventromedial medulla co-regulate motor and sympathetic tone.

Applications

Unspecified application

Species

Unspecified reactive species

Zicong Zhang,Junfeng Su,Jing Tang,Leeyup Chung,Jessica C Page,Carla C Winter,Yuchu Liu,Evgenii Kegeles,Sara Conti,Yu Zhang,Jason Biundo,Joshua I Chalif,Charles Y Hua,Zhiyun Yang,Xue Yao,Yang Yang,Shuqiang Chen,Jan M Schwab,Kuan Hong Wang,Chinfei Chen,Michael J Prerau,Zhigang He

Nature plants 10:283-299 PubMed38278950

2024

O-glycosylation of the transcription factor SPATULA promotes style development in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Jiang,Seamus Curran-French,Samuel W H Koh,Iqra Jamil,Benguo Gu,Luca Argirò,Sergio G Lopez,Carlo Martins,Gerhard Saalbach,Laila Moubayidin

Blood advances 8:725-731 PubMed38147622

2023

R-Spondin1 protects gastric stem cells and mitigates gastric GVHD in allogeneic hematopoietic stem cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Eiko Hayase,Takahide Ara,Yumika Saito,Shuichiro Takahashi,Kosuke Yoshioka,Hiroyuki Ohigashi,Reiki Ogasawara,Emi Yokoyama,Tomohiro Yamakawa,Ko Ebata,Yuta Hasegawa,Kazuma Tomizuka,Takanori Teshima

Nature 624:403-414 PubMed38092914

2023

A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Carla C Winter,Anne Jacobi,Junfeng Su,Leeyup Chung,Cindy T J van Velthoven,Zizhen Yao,Changkyu Lee,Zicong Zhang,Shuguang Yu,Kun Gao,Geraldine Duque Salazar,Evgenii Kegeles,Yu Zhang,Makenzie C Tomihiro,Yiming Zhang,Zhiyun Yang,Junjie Zhu,Jing Tang,Xuan Song,Ryan J Donahue,Qing Wang,Delissa McMillen,Michael Kunst,Ning Wang,Kimberly A Smith,Gabriel E Romero,Michelle M Frank,Alexandra Krol,Riki Kawaguchi,Daniel H Geschwind,Guoping Feng,Lisa V Goodrich,Yuanyuan Liu,Bosiljka Tasic,Hongkui Zeng,Zhigang He

The New phytologist 240:224-241 PubMed37424336

2023

Increasing amyloplast size in wheat endosperm through mutation of PARC6 affects starch granule morphology.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Esch,Qi Yang Ngai,J Elaine Barclay,Rose McNelly,Sadiye Hayta,Mark A Smedley,Alison M Smith,David Seung
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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