AB48653

Biotin Anti-S100 antibody

Name: Biotin Anti-S100 antibody (ab48653) | Abcam
Brand: Abcam Limited
SKU: AB48653

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(1 Publication)

Rabbit Polyclonal S10A1 antibody - conjugated to Biotin. Suitable for IP, ELISA, WB, RIA, EIA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human S100A1.

View Alternative Names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

EIA, IP, ELISA, RIA, WB

applications

Immunogen

Native Full Length Protein corresponding to Human S100A1.

P23297

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.25% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S100 protein family consists of low-molecular-weight proteins with individual members like S100B and S100A4 each having distinct roles. S100 proteins range typically between 9 to 13 kDa in mass. They are expressed broadly but especially in the central nervous system where glial cells are an important source and in several epithelial tissues including melanocytes. These proteins are noted for their calcium-binding properties facilitated by EF-hand motifs influencing both intracellular and extracellular processes.

Biological function summary

S100 proteins drive diverse cellular functions such as regulation of cell cycle differentiation and motility. They often operate as dimers and participate in the formation of protein complexes impacting cellular signaling networks. By binding calcium S100 proteins undergo conformational changes that allow interaction with target proteins modulating processes like cytoskeleton dynamics and neuronal survival.

Pathways

S100 proteins are significant in the regulation of the MAPK/ERK and NF-kB pathways. These pathways are critical for cell proliferation apoptosis and response to environmental stress. S100B in particular interacts with the receptor for advanced glycation end-products (RAGE) influencing downstream signaling cascades and amplifying the cellular response to inflammatory stimuli.

S100 proteins are implicated in the progression of conditions such as melanoma and Alzheimer's disease. In melanoma S100B levels serve as a biomarker reflecting disease progression potentially interacting with p53 to diminish its tumor suppressor function. In Alzheimer's disease S100B overexpression correlates with neuroinflammation and neuronal damage highlighting its association with disease through pathways involving amyloid-beta and tau proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).

See full target information S100A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed36802340

2023

Neutrophil extracellular traps contribute to coagulopathy after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Jin,Fang Wang,Jiawei Tian,Xinyi Zhao,Jiawei Dong,Nan Wang,Zhihui Liu,Hongtao Zhao,Wenqiang Li,Ge Mang,Shaoshan Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-S100 antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise