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AB284658

Biotin Anti-SARS-CoV-2 envelope protein antibody

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(2 Publications )

Anti-SARS-CoV-2 envelope protein antibody conjugated to Biotin is a rabbit polyclonal antibody suitable for ELISA.
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ELISA - Biotin Anti-SARS-CoV-2 envelope protein antibody (AB284658)
  • ELISA

Supplier Data

ELISA - Biotin Anti-SARS-CoV-2 envelope protein antibody (AB284658)

SARS envelope peptide, 10 μg/mL, incubate at 4°C overnight.

Detection Antibodies : SARS-CoV-2 Spike antibody, ab284658, dilution : 0.5-1000 ng/mL, incubate at room temperature for 1 hr.

by HRP-conjugated streptavidin at 1/10,000, incubate at room temperature for 1 hr.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

SARS-CoV-2

Applications

ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 99.9698% Water, 0.00016% Sodium chloride, 0.00003% disodium hydrogen phosphate, 0.00001% Potassium chloride, 0.00001% Sodium dihydrogen phosphate
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 envelope protein also known as E protein is a small membrane protein with an approximate mass of 8.4 kDa. This protein integrates into the membrane of the virus and plays significant roles during the viral life cycle particularly in assembly and budding. Researchers identified its presence in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) where it participates in the formation of the viral envelope.
Biological function summary

The envelope protein acts as a viroporin forming ion channels that modulate the ionic environment necessary for the virus. It partakes in the viral assembly process and influences virus morphology. E protein also works in a complex with other structural proteins like the membrane (M) protein contributing to the virus's ability to infect host cells. This complex facilitates proper viral particle assembly and release.

Pathways

The envelope protein interacts with various cellular pathways that affect the host's immune response. It modulates pathways involved in inflammasome activation and cytokine production. For instance it affects NLRP3 inflammasome pathway which has close interactions with M protein and other viral components. By disrupting these pathways the envelope protein helps in avoiding host immune detection and promoting viral pathogenicity.

The envelope protein relates to COVID-19 and its associated respiratory syndromes. Its role in the assembly and release of the virus links it to the severe respiratory symptoms seen in COVID-19 patients. Additionally this protein has connections to the host immune response interacting with proteins like the N protein which is involved in modulating severe inflammatory responses observed in COVID-19 cases. This makes the envelope protein a potential target for antiviral therapies and vaccine development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed : 33229438). May act by slowing down the cell secretory pathway (PubMed : 33229438). May interfere with tight-junction stability by interacting with host MPP5. This would result in disruption of epithelial barriers, thereby amplifying inflammatory processes (PubMed : 32891874).
See full target information SARS-CoV-2 envelope protein

Alternative Names

4, E, Envelope small membrane protein, sM protein
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Journal of controlled release : official journal of the Controlled Release Society 362:536-547 PubMed37648082

2023

Peptide delivery of a multivalent mRNA SARS-CoV-2 vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Cian M McCrudden,Lindsey Bennie,Philip Chambers,Jordan Wilson,Megan Kerr,Monika Ziminska,Hayley Douglas,Sarah Kuhn,Emma Carroll,Garrett O'Brien,Niamh Buckley,Nicholas J Dunne,Helen O McCarthy

Nature communications 14:5077 PubMed37604832

2023

CD36 mediates SARS-CoV-2-envelope-protein-induced platelet activation and thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Tang,Yanyan Xu,Yun Tan,Hui Shi,Peipei Jin,Yunqi Li,Jialin Teng,Honglei Liu,Haoyu Pan,Qiongyi Hu,Xiaobing Cheng,Junna Ye,Yutong Su,Yue Sun,Jianfen Meng,Zhuochao Zhou,Huihui Chi,Xuefeng Wang,Junling Liu,Yong Lu,Feng Liu,Jing Dai,Chengde Yang,Saijuan Chen,Tingting Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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