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AB284654

Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a]

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Rabbit Recombinant Monoclonal NCAP antibody - conjugated to Biotin. Suitable for ELISA, sELISA and reacts with Recombinant full length protein - SARS-CoV-2 samples.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

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ELISA - Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284654)
  • ELISA

Supplier Data

ELISA - Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284654)

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL.

ab284654 was serially diluted 1/2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.

Sandwich ELISA - Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284654)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284654)

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies : 84C4a as a capture antibody and ab284654 (75G5a-biotin) as a detection antibody. COVID-19 NP was serially diluted 1/2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and ab284654 (75G5a-biotin) detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).

Sandwich ELISA - Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284654)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a] (AB284654)

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies : 85C1 as a capture antibody and ab284654 (75G5a-biotin) as a detection antibody. COVID-19 NP was serially diluted 1/2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and ab284654 (75G5a-biotin) detected COVID-19 NP antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).

  • Unconjugated

    Anti-SARS-CoV-2 nucleocapsid protein antibody [75G5a]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

75G5a

Isotype

IgG

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

SARS-CoV-2

Applications

sELISA, ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - SARS-CoV-2": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.00014-1 µg/mL", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>50 - 12000 pg/mL</p>" }, "SARS-CoV-2": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.
See full target information N
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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