Goat Polyclonal SDF1 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CXCL12 aa 1 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CXCL12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48061
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Recombinant fragment - Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 0.10000-0.20000 µg/mL | Notes When used in conjunction with compatible secondary reagents, the detection limit for recombinant human SDF1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).
Alternative names
SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF
Recommended products
Goat Polyclonal SDF1 antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CXCL12 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Conjugation
- Biotin
- Storage buffer
Constituents: PBS, 0.1% BSA
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CXCL12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48061
- Purification technique
- Affinity purification
- Reconstitution
- Reconstitute in PBS, 0.1% BSA
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SDF1 beta also known as CXCL12b or stromal cell-derived factor 1 beta is a small chemokine with a mass around 8 kDa. SDF1 beta plays a significant role as a signaling molecule involved in mediating various cellular responses. Expression of this chemokine occurs in a variety of tissues including spleen brain liver and skeletal muscle. Its mechanical function mainly involves binding to the CXCR4 receptor on target cells leading to the activation of signaling cascades that modulate cellular behavior.
- Biological function summary
SDF1 beta participates in the regulation of immune cell trafficking and homing. As a prominent chemokine it directs leukocytes to sites where they perform their immunological duties such as inflamed or damaged tissue. Although it generally acts independently SDF1 beta can be part of a larger chemokine network collaborating with other chemokines and cytokines to orchestrate coordinated migration and positioning of immune cells.
- Pathways
SDF1 beta is intricately linked with the chemokine signaling pathway and homing of hematopoietic stem cells. Activation of its pathway involves the prominent receptor CXCR4 which mediates signals that are important for the directional movement of these cells. SDF1 beta plays a central role in the hematopoiesis process where it interacts with molecules like CXCR7 which sometimes acts as a supportive player for its receptor influencing the hematopoietic stem cell niche regulation as well.
- Associated diseases and disorders
Involvement of SDF1 beta has been identified in cancer progression and inflammatory diseases like rheumatoid arthritis. In cancer it contributes to tumor growth and metastasis with CXCR4 being its main receptor partner. In rheumatoid arthritis overexpression or dysregulation of SDF1 beta influences the infiltration and retention of immune cells in the joints exacerbating inflammation. Understanding these interactions provides insights for potential therapeutic approaches targeting the SDF1 beta and CXCR4 axis in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Biotin Anti-SDF1 beta antibody (ab271279)
To detect Human SDF-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1 beta, respectively.
Non-reducing conditions.
All lanes: Western blot - Biotin Anti-SDF1 beta antibody (ab271279)
Predicted band size: 11 kDa
Sandwich ELISA - Biotin Anti-SDF1 beta antibody (ab271279)
Human SDF1 betawas detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271279.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant SDF1 beta protein.
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