AB95644

Biotin Anti-SIRP alpha antibody [P84]

Name: Biotin Anti-SIRP alpha antibody [P84] (ab95644) | Abcam
Brand: Abcam Limited
SKU: AB95644
Price: 275 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rat Monoclonal SIRP alpha antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Tissue preparation containing Sirpa protein.

View Alternative Names

CD172a, Bit, Myd1, Ptpns1, Shps1, Sirp, Sirpa, Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MyD-1 antigen, Signal-regulatory protein alpha-1, p84, Sirp-alpha-1, mSIRP-alpha1

1 Images

Flow Cytometry - Biotin Anti-SIRP alpha antibody [P84] (AB95644)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-SIRP alpha antibody [P84] (AB95644)

Staining of C57BL/6 bone marrow cells with with Anti-Mouse CD11b APC and 0.125 µg of ab95644, follwed by Streptavidin PE. Cells in the lymphoid (left) or myeloid (right) gates were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

P84

Isotype

IgG1

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Tissue preparation containing Sirpa protein. The exact immunogen used to generate this antibody is proprietary information.

P97797

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.125-0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>A final volume of 100 μl is recommended for staining the cell sample.</p><p><a href='/en-us/products/primary-antibodies/biotin-rat-igg1-kappa-rtk2071-isotype-control-ab18403'>ab18403</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

ab95644 has been reported to have neutralizing activity.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIRP alpha also known as CD172a is a transmembrane receptor protein with a mass ranging between 70-110 kDa due to glycosylation. This protein extensively expresses on the surface of myeloid cells neurons and a subset of T-cells and is part of the immunoglobulin superfamily. SIRP alpha interacts with its ligand CD47 a widely expressed glycoprotein involved in immune response regulation. Its mechanical action primarily involves signal regulation through the recruitment of SHP-1 and SHP-2 two cytoplasmic tyrosine phosphatases.

Biological function summary

SIRP alpha functions significantly in the regulation of phagocytosis acting as a "don't eat me" signal to macrophages upon binding with CD47. It does not act alone; rather it is part of a complex that recruits SHP-1 and SHP-2 leading to inhibition of dephosphorylation activities essential for engulfment processes. This regulatory mechanism is important for maintaining cellular homeostasis ensuring that healthy cells are not mistakenly destroyed by the immune system.

Pathways

SIRP alpha plays an important role in the innate immune pathways involving the regulation of phagocytosis and cell-cell adhesion. Particularly it fits into the immune checkpoint pathways where it interacts closely with proteins like CD47 and plays a role in the interaction between the immune system and cancer cells. Through these pathways SIRP alpha helps maintain balance in the immune response allowing for the recognition of self versus non-self therefore preventing autoimmunity while facilitating the clearance of pathogens.

SIRP alpha is implicated in cancer and autoimmune diseases. In cancer its interaction with CD47 allows tumor cells to escape phagocytosis promoting tumor survival and growth. In autoimmune disorders dysregulated SIRP alpha expression or signaling could miscommunicate immune signals leading to the destruction of healthy tissue. Understanding the link between SIRP alpha and these conditions can reveal potential targets for therapeutic development especially using inhibitors or modulators targeting the SIRP alpha-CD47 interaction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunoglobulin-like cell surface receptor for CD47 (PubMed : 18045614). Acts as a docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity). Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (PubMed : 30726215). Receptor for THBS1 (By similarity). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (By similarity).

See full target information Sirpa

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 172:4091-9 PubMed15034021

2004

Src homology 2 domain-containing protein tyrosine phosphatase substrate 1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes.

Applications

Flow Cyt

Species

Unspecified reactive species

Atsushi Fukunaga,Hiroshi Nagai,Tetsuya Noguchi,Hideki Okazawa,Takashi Matozaki,Xijun Yu,Carl F Lagenaur,Nakayuki Honma,Masamitsu Ichihashi,Masato Kasuga,Chikako Nishigori,Tatsuya Horikawa

Developmental biology 137:219-32 PubMed2303162

1990

Central nervous system antigen P84 can serve as a substrate for neurite outgrowth.

Applications

Unspecified application

Species

Unspecified reactive species

W Chuang,C F Lagenaur

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-SIRP alpha antibody [P84]

Flow Cytometry - Biotin Anti-SIRP alpha antibody [P84] (AB95644)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise