AB198366

Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB198366)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB198366)

IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab198366, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
HRP

HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
578 PE

PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
660 APC

APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
Unconjugated

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
Carrier free

Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1845Y

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Tilapia": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.

Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1616519 PubMed41059303

2025

Targeting RAB7 in human B lymphoma by a small molecule inhibitor arrests tumor cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Fernandez,Rui Wang,Jingwei Wang,Shuai Wu,Kenneth Holder,Alia Nazarullah,Ricardo C T Aguiar,Zijun Y Xu-Monette,Ken H Young,Hui Yan,Zhenming Xu

Military Medical Research 9:58 PubMed36229865

2022

Cardiac-targeted PIASy gene silencing mediates deSUMOylation of caveolin-3 and prevents ischemia/reperfusion-induced Na1.5 downregulation and ventricular arrhythmias.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Chen Hu,Xin Wei,Jin-Min Liu,Lin-Lin Han,Cheng-Kun Xia,Jing Wu,Tao You,A-Fang Zhu,Shang-Long Yao,Shi-Ying Yuan,Hao-Dong Xu,Zheng-Yuan Xia,Ting-Ting Wang,Wei-Ke Mao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB198366)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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