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AB95647

Biotin Anti-TCR alpha + TCR beta antibody [IP26]

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(1 Publication)

Mouse Monoclonal TRAC antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human TRBV7-9.

View Alternative Names

TCRA, TRAC, T cell receptor alpha chain constant

1 Images
Flow Cytometry - Biotin Anti-TCR alpha + TCR beta antibody [IP26] (AB95647)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-TCR alpha + TCR beta antibody [IP26] (AB95647)

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC, CD19 PE and TCR alpha/beta Biotin followed by staining with APC conjugated streptavidin. As expected based on known relative expression patterns, TCR alpha/beta clone IP26 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IP26

Isotype

IgG1

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human TRBV7-9. The exact immunogen used to generate this antibody is proprietary information.

P04435

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>0.5 μg/test</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The T-cell receptor (TCR) alpha and TCR beta sometimes referred to as alpha beta TCR or alfa TCR are essential components of the immune response machinery. TCR alpha and beta chains each have a mass that varies between 30-40 kDa depending on the variable region's length. These receptor chains are expressed on the surface of T-cells more specifically on the vast majority of T lymphocytes and play a critical role in antigen recognition. Commercial names like anti-TCR or H57 might refer to antibodies targeting these chains.
Biological function summary

TCR alpha and beta chains form a heterodimeric complex with other important proteins like CD3 to transmit signals inside the T-cell upon antigen binding. This complex becomes active when T-cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. The interaction between TCR and MHC-peptide complex initializes T-cell activation and subsequent immune responses being central to adaptive immunity.

Pathways

TCR alpha and beta are integral to the T-cell receptor signaling pathway. This pathway facilitates the activation of important transcription factors such as NF-kB and AP-1 which drive T-cell proliferation and differentiation. The signaling involves several other proteins such as protein kinase C theta and ZAP-70 which further propagate intracellular signaling cascades essential for a fully functional immune response.

Mutations or malfunctions in TCR alpha and beta chains can associate with autoimmune disorders like rheumatoid arthritis and certain types of immunodeficiency. Rheumatoid arthritis involves aberrant TCR signaling pathways that result in inappropriate immune responses against self-antigens. In some immunodeficiencies defective TCR signaling impairs T-cell development and function potentially involving related proteins like CD3 and ZAP-70 leading to compromised immune defenses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of T cell receptor (TR) alpha chain (PubMed : 24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed : 25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed : 23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed : 15040585).
See full target information TRAC

Additional targets

TRBC2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 172:4091-9 PubMed15034021

2004

Src homology 2 domain-containing protein tyrosine phosphatase substrate 1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Fukunaga,Hiroshi Nagai,Tetsuya Noguchi,Hideki Okazawa,Takashi Matozaki,Xijun Yu,Carl F Lagenaur,Nakayuki Honma,Masamitsu Ichihashi,Masato Kasuga,Chikako Nishigori,Tatsuya Horikawa
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