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AB25209

Biotin Anti-TCR gamma + TCR delta antibody [UC7-13D5]

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(3 Publications)

Armenian hamster Monoclonal CD3d antibody - conjugated to Biotin. Suitable for IP, Flow Cyt, FuncS, IHC-Fr and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

CD3d, T3d, Cd3d, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD3g, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, Cd3g

  • Unconjugated

    Anti-TCR gamma + TCR delta antibody [UC7-13D5]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

UC7-13D5

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr, IP, Flow Cyt, FuncS

applications

Specificity

ab25209 is specific for the gamma-delta heterodimer and plate-bound ab25209 activates gamma-delta TCR bearing cells. This antibody does not react with alpha-beta TCR-expressing T cells.

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Alternative Products

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Primary Antibodies

AB25663

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Primary Antibodies

AB183685

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>In vitro activation of gamma-delta TCR-bearing cells and depletion of gamma-delta TCR-expressing cells.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCR gamma + TCR delta also known as gamma delta T-cell receptor is a part of the T-cell receptor (TCR) family. It has alternative names like gammadelta TCR or simply TCRδ/γ. The gamma and delta chains form a heterodimer that is part of the cell surface receptor complex. This complex is important for ligand recognition and immune response. The mass of the TCR gamma and TCR delta chains is not specified but they form between ZAP-70 and the CD3 complex on the surface of a subset of T-cells. These receptors are mainly expressed on gamma delta T-cells which are a minor population of T-cells in peripheral blood but more abundant in mucosal tissues and epithelial layers.
Biological function summary

TCR gamma and TCR delta are involved in recognizing antigens without presentation by major histocompatibility complex (MHC) molecules. The receptor complex allows these cells to respond to a diverse range of non-peptide antigens mostly derived from microbial origin including phosphoantigens and lipids. This enables gamma delta T-cells to bridge innate and adaptive immunity. They do not always require antigen processing and can recognize antigens directly contributing efficiently to immune surveillance and early response to infections and cellular stress.

Pathways

TCR gamma and TCR delta are important in the signaling networks that mediate immune responses. They are involved in pathways such as the nuclear factor of activated T-cells (NFAT) and mitogen-activated protein kinase (MAPK) pathways. These pathways promote the activation proliferation and differentiation of T-cells upon antigen recognition. The TCR complex including CD3 plays a role in these signal transduction events and ZAP-70 a protein tyrosine kinase is key for initiating these pathways upon TCR activation.

Mutations or dysregulation in TCR gamma and TCR delta can lead to immune-related conditions. For example abnormalities in these receptors have been linked to autoimmune diseases such as celiac disease and multiple sclerosis. Their role in these disorders is related to faulty recognition and response to autoantigens leading to inappropriate immune responses. Additionally gamma delta T-cells and their receptors are of interest in cancer research as they participate in tumor immunosurveillance. Connections with molecules like CD3 and ZAP-70 facilitate ongoing exploration for therapeutic interventions targeting immune-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed : 10935641). Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.
See full target information Cd3d

Additional targets

T-cell surface glycoprotein CD3 gamma chain
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 10:831 PubMed36035005

2022

The effects of photobiomodulation therapy on inflammatory mediators, immune infiltration, and angiogenesis in a mouse model of rosacea.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwei Wu,Yaoxi Su,Lian Wang,Bensen Sun,Xian Jiang

Evidence-based complementary and alternative medicine : eCAM 2020:3056797 PubMed32280354

2020

Gubenzhike Recipe Ameliorates Respiratory Mucosal Immunity in Mice with Chronic Obstructive Pulmonary Disease through Upregulation of the T Lymphocytes and KGF Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Yue-Qi Wang,Qiang Liao,Shi-Huan Tang,Zhe Cai,Hong-Chun Zhang

Nature 343:714-9 PubMed1968227

1990

Self-reactive gamma delta T cells are eliminated in the thymus.

Applications

Unspecified application

Species

Unspecified reactive species

A L Dent,L A Matis,F Hooshmand,S M Widacki,J A Bluestone,S M Hedrick
View all publications
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