Rabbit Polyclonal TGF beta 3 antibody - conjugated to Biotin. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TGFB3 aa 300 to C-terminus.
IgG
Rabbit
Biotin
Constituents: PBS, 0.1% BSA
Lyophilized
Polyclonal
WB | |
---|---|
Recombinant fragment - Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 0.10000-0.20000 µg/mL | Notes - |
Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.Latency-associated peptideRequired to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3 (By similarity). Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3 (By similarity).Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus (By similarity). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3 (By similarity). Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
Transforming growth factor beta-3 proprotein, TGFB3
Rabbit Polyclonal TGF beta 3 antibody - conjugated to Biotin. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TGFB3 aa 300 to C-terminus.
Transforming growth factor beta-3 proprotein, TGFB3
IgG
Rabbit
Biotin
Constituents: PBS, 0.1% BSA
Lyophilized
Polyclonal
Affinity purification
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
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Full details and terms and conditions can be found here:
Terms & Conditions.
Western blot analysis of varying levels of Recombinant human TGF-β3 protein using ab271246 at 0.1 μg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant TGF-β3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24
All lanes: Western blot - Biotin Anti-TGF beta 3 antibody (ab271246)
Predicted band size: 47 kDa
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