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AB271211

Biotin Anti-TNF Receptor I antibody

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(1 Publication)

Rabbit Polyclonal TNF Receptor I antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF1A aa 1-250.

View Alternative Names

CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

2 Images
Sandwich ELISA - Biotin Anti-TNF Receptor I antibody (AB271211)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-TNF Receptor I antibody (AB271211)

Human TNF receptor 1 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271211. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-Receptor 1 protein.

Western blot - Biotin Anti-TNF Receptor I antibody (AB271211)
  • WB

Supplier Data

Western blot - Biotin Anti-TNF Receptor I antibody (AB271211)

Western blot analysis of varying levels of Recombinant human TNF Receptor I protein using ab271211 at 0.2 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF Receptor I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24

All lanes:

Western blot - Biotin Anti-TNF Receptor I antibody (ab271211)

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

sELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF1A aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P19438

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in PBS, 0.1% BSA
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
See full target information TNFRSF1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical laboratory analysis 36:e24232 PubMed34995016

2022

A combined biomarker panel shows improved sensitivity and specificity for detection of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Mao,Yong Tang,Ming-Jing Deng,Chun-Tao Huang,Dong Lan,Wen-Zheng Nong,Li Li,Qi Wang
View all publications
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Product promise

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