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AB271257

Biotin Anti-TPO antibody

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Rabbit Polyclonal Thrombopoietin antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human THPO aa 1-200.

View Alternative Names

MGDF, THPO, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML

2 Images
ELISA - Biotin Anti-TPO antibody (AB271257)
  • ELISA

Unknown

ELISA - Biotin Anti-TPO antibody (AB271257)

Human TPO was detected by sandwich ELISA (using 100 μl/well) using a concentration of 0.25-1.0 μg/ml of ab271257. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant TPO protein.

Western blot - Biotin Anti-TPO antibody (AB271257)
  • WB

Supplier Data

Western blot - Biotin Anti-TPO antibody (AB271257)

Western blot analysis of varying levels of Recombinant human TPO protein using ab271257 at 0.1 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant TPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24

All lanes:

Western blot - Biotin Anti-TPO antibody (ab271257)

Predicted band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

WB, ELISA

applications

Immunogen

Recombinant Fragment Protein within Human THPO aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P40225

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Reactivity data

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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Purification technique
Affinity purification
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroid peroxidase (TPO) also known as thyroid microsomal antigen is an essential enzyme involved in the biosynthesis of thyroid hormones. TPO mechanistically catalyzes the iodination of tyrosine residues in thyroglobulin and the coupling of iodotyrosines to produce thyroxine (T4) and triiodothyronine (T3). This enzyme has a molecular mass approximately of 105 kDa and expresses mainly in the thyroid gland. Researchers often use anti-TPO antibodies to study this protein in scientific investigations because of its significant role in thyroid hormone production.
Biological function summary

TPO is integral in facilitating the thyroid gland's production of hormones necessary for metabolism. It operates in the thyroid follicular cells and works in conjunction with other components like hydrogen peroxide to ensure efficient hormone synthesis. TPO is not part of a larger complex but acts closely with the dual oxidase system that provides the hydrogen peroxide required for its enzymatic activity. This relationship is important for maintaining optimal levels of thyroid hormones necessary for various physiological functions.

Pathways

The biosynthesis of thyroid hormones is the primary pathway involving TPO. TPO plays a role along with thyroid-stimulating hormone receptor (TSHR) to regulate hormone synthesis and release. Another pathway the iodine metabolism pathway incorporates TPO's function to ensure that iodine an essential element effectively integrates into thyroglobulin. This integration is facilitated with the help of the sodium/iodide symporter (NIS) which allows the active transport of iodide ions into the thyroid cells.

TPO is closely related to autoimmune thyroid diseases (AITD) such as Hashimoto's thyroiditis and Graves' disease. These conditions often see the presence of anti-TPO antibodies which indicate an autoimmune response against TPO leading to thyroid dysfunction. A connection to these disorders can also involve the protein thyroglobulin (Tg) as both TPO and Tg are critical in thyroid hormone synthesis and are targets for autoantibodies in AITD. Understanding TPO's role helps in diagnosing and managing thyroid disorders effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
See full target information THPO
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Product promise

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