AB95531

Biotin Anti-TRAIL antibody [N2B2]

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(2 Publications)

Rat Monoclonal TRAIL antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Tnfsf10 protein.

View Alternative Names

CD253, Trail, Tnfsf10, Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

1 Images

Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (AB95531)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (AB95531)

ab95531, at 0.125 µg, staining of TRAIL transfected cells (filled) or with Rat IgG2a Isotype Control (open) followed by Streptavidin PE by Flow Cytometry. Total viable cells were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

N2B2

Isotype

IgG2a

Light chain type

kappa

Conjugation

Biotin

Excitation/Emission

Carrier free

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Tnfsf10 protein. The exact immunogen used to generate this antibody is proprietary information.

P50592

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.125-0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>in a final volume of 100 μL.</p><p><a href='/en-us/products/primary-antibodies/biotin-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18445'>ab18445</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

ab95531 inhibits IL2- or IL15-activated NK cell cytotoxicity against Mouse fibrosarcoma L929 target cells.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.

Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

See full target information Tnfsf10

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 172:123-9 PubMed14688317

2003

NK cell TRAIL eliminates immature dendritic cells in vivo and limits dendritic cell vaccination efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Hayakawa,Valentina Screpanti,Hideo Yagita,Alf Grandien,Hans-Gustaf Ljunggren,Mark J Smyth,Benedict J Chambers

Journal of immunology (Baltimore, Md. : 1950) 163:1906-13 PubMed10438925

1999

Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells.

Applications

Flow Cyt

Species

Mouse

N Kayagaki,N Yamaguchi,M Nakayama,K Takeda,H Akiba,H Tsutsui,H Okamura,K Nakanishi,K Okumura,H Yagita

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-TRAIL antibody [N2B2]

Flow Cytometry - Biotin Anti-TRAIL antibody [N2B2] (AB95531)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise