AB34673

Biotin Anti-Transferrin antibody - Serum Loading Control

Name: Anti-Transferrin Biotin antibody (ab34673) Rabbit polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB34673
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Transferrin antibody - conjugated to Biotin. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human TF.

View Alternative Names

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

3 Images

Supplier Data

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (AB34673)

All lanes:

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (ab34673) at 1/1000 dilution

All lanes:

Human depleted serum

Secondary

All lanes:

Peroxidase streptavidin conjugate at 1/40000 dilution

Predicted band size: 77 kDa

false

Supplier Data

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (AB34673)

Multiplex western blot analysis of 35 μg of human serum with 350 ng of added GST. Green bands are labelling human transferrin, with ab34673 and a donkey anti-rabbit DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a donkey anti-goat DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a donkey anti-mouse DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 30 minutes at 4°C.

All lanes:

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (ab34673)

Predicted band size: 46 kDa,77 kDa

false

Supplier Data

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (AB34673)

Multiplex western blot analysis of human serum. Green bands are labelling human transferrin, with ab34673 and a DyLight® 549 conjugate. Red bands are labelling trypsin, with ab7633 and a DyLight® 649 conjugate. Blue bands are labelling human IgG with a primary and a DyLight® 488 conjugate. All primaries were diluted to 1/1,000 and incubated overnight at 4°C. All secondaries were diluted to 1/10,000 for 1 hour at 4°C.

All lanes:

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (ab34673)

Predicted band size: 46 kDa,77 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Full Length Protein corresponding to Human TF. The exact immunogen used to generate this antibody is proprietary information.

P02787

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

10-20 Biotin molecules per rabbit IgG molecule.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification technique

Ion exchange chromatography

Purification notes

Anti-Transferrin Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.

Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.

See full target information TF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nanomaterials (Basel, Switzerland) 8: PubMed30562983

2018

Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Hauser,Manuela Estermann,Ana Milosevic,Lukas Steinmetz,Dimitri Vanhecke,Dedy Septiadi,Barbara Drasler,Alke Petri-Fink,Vincent Ball,Barbara Rothen-Rutishauser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Transferrin antibody - Serum Loading Control

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (AB34673)

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (AB34673)

Western blot - Biotin Anti-Transferrin antibody - Serum Loading Control (AB34673)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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