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Goat Polyclonal TRAP/CD40L antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus.

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Images

Western blot - Biotin Anti-TRAP/CD40L antibody (AB271210), expandable thumbnail
  • Sandwich ELISA - Biotin Anti-TRAP/CD40L antibody (AB271210), expandable thumbnail

Key facts

Isotype

IgG

Host species

Goat

Conjugation

Biotin

Storage buffer

Constituents: PBS, 0.1% BSA

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29965

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBsELISA
Recombinant full length protein - Human
Tested
Tested

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

0.10000-0.20000 µg/mL

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Target data

Function

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).CD40 ligand, soluble formActs as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Alternative names

Recommended products

Goat Polyclonal TRAP/CD40L antibody - conjugated to Biotin. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus.

Key facts

Isotype

IgG

Conjugation

Biotin

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29965
Purification technique

Affinity purification

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

Associated diseases and disorders

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

Product promise

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2 product images

  • Western blot - Biotin Anti-TRAP/CD40L antibody (ab271210), expandable thumbnail

    Western blot - Biotin Anti-TRAP/CD40L antibody (ab271210)

    Western blot analysis of varying levels of Recombinant human TRAP/CD40L protein using ab271210 at 0.2 μg/ml.

    Used in conjunction with compatible secondary reagents the detection limit for recombinant TRAP/CD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Lane ng/lane
    1 Marker
    2 250
    3 125
    4 62.5
    5 31.25
    6 15.625
    7 7.8
    8 3.9
    9 1.95
    10 0.975
    11 0.4875
    12 0.24

    All lanes: Western blot - Biotin Anti-TRAP/CD40L antibody (ab271210)

    Predicted band size: 29 kDa

  • Sandwich ELISA - Biotin Anti-TRAP/CD40L antibody (ab271210), expandable thumbnail

    Sandwich ELISA - Biotin Anti-TRAP/CD40L antibody (ab271210)

    Human TRAP/CD40L was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271210. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTRAP/CD40L protein.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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