AB84247

Biotin Anti-TSLP antibody

Name: Biotin Anti-TSLP antibody (ab84247) | Abcam
Brand: Abcam Limited
SKU: AB84247
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal TSLP antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TSLP.

View Alternative Names

Thymic stromal lymphopoietin, TSLP

2 Images

Sandwich ELISA - Biotin Anti-TSLP antibody (AB84247)

sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-TSLP antibody (AB84247)

To detect human TSLP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Rabbit polyclonal to TSLP (ab47943) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human TSLP.

Supplier Data

Western blot - Biotin Anti-TSLP antibody (AB84247)

All lanes : Anti-TSLP antibody (ab84247) at 0.2 μg/ml

Lane 1 : Marker

Unreduced Recombinant Human TSLP protein (ab50020) :

Lane 2 : 250 ng

Lane 3 : 125 ng

Lane 4 : 62.5 ng

Lane 5 : 31.25 ng

Lane 6 : 15.625 ng

Lane 7 : 7.8 ng

Lane 8 : 3.9 ng

Lane 9 : 1.95 ng

Lane 10 : 0.975 ng

Lane 11 : 0.4875 ng

Lane 12 : 0.24 ng

All lanes:

Western blot - Biotin Anti-TSLP antibody (ab84247)

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Excitation/Emission

Carrier free

Yes

Reacts with

Human

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TSLP.

Assay Kits

AB192149

Human TSLP ELISA Kit

SimpleStep

ELISA - Human TSLP ELISA Kit (AB192149)

Assay Kits

AB192149

Human TSLP ELISA Kit

SimpleStep

ELISA - Human TSLP ELISA Kit (AB192149)

Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

Primary Antibodies

AB83163

Biotin Anti-FGF1 antibody

Sandwich ELISA - Biotin Anti-FGF1 antibody (AB83163)

Primary Antibodies

AB272303

Anti-CD127 antibody [EPR22835-39] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CD127 antibody [EPR22835-39] - BSA and Azide free (Capture) (AB272303)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "The detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "Use 100 μl/well antibody solution. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTSLP." }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "The detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).

Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Isoform 1. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.. Isoform 2. May act as an antimicrobial peptide in the oral cavity and on the skin.

See full target information TSLP

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0117038 PubMed25643281

2015

Antimicrobial protein and Peptide concentrations and activity in human breast milk consumed by preterm infants at risk of late-onset neonatal sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Trend,Tobias Strunk,Julie Hibbert,Chooi Heen Kok,Guicheng Zhang,Dorota A Doherty,Peter Richmond,David Burgner,Karen Simmer,Donald J Davidson,Andrew J Currie

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com