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AB27672

Biotin Anti-V5 tag antibody [SV5-Pk1]

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Anti-V5 tag antibody [SV5-Pk1] - Biotin conjugated (ab27672) is a mouse monoclonal antibody detecting V5 tag in Western Blot, IHC-Fr, ELISA.

- Trusted since 2005

View Alternative Names

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SV5-Pk1

Isotype

IgG2a

Conjugation

Biotin

Excitation/Emission
Carrier free

No

Applications

WB, IHC-Fr, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Ab27672 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.

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Reactivity data

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Product details

What is this antibody validated in?
Biotin Anti-V5 tag antibody [SV5-Pk1] (ab27672) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr), ELISA. Tagged protein detection
V5 tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone SV5-Pk1 is also available pre-conjugated to a variety of labels for your convenience – Anti-V5 tag Biotin [SV5-Pk1] (ab27672).

Other related products
We have a range of other formats of antibody clone [SV5-Pk1] also available for your convenience: ab27671, Biotin - ab27672
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
This antibody was purified from tissue culture supernatant by affinity chromatography.
Storage buffer
Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information V5 tag
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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