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Rabbit Polyclonal VEGFB antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VEGFB.

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Images

Western blot - Biotin Anti-VEGFB antibody (AB233505), expandable thumbnail
  • Western blot - Biotin Anti-VEGFB antibody (AB233505), expandable thumbnail
  • Sandwich ELISA - Biotin Anti-VEGFB antibody (AB233505), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Biotin

Storage buffer

Constituents: PBS

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human VEGFB. Database link P49765

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBsELISA
Human
Predicted
Predicted
Recombinant full length protein - Human
Tested
Tested

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

0.10000-0.20000 µg/mL

Notes

-

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

0.25000-1.00000 µg/mL

Notes

-

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

Alternative names

Recommended products

Rabbit Polyclonal VEGFB antibody - conjugated to Biotin. Carrier free. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VEGFB.

Key facts

Isotype

IgG

Conjugation

Biotin

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human VEGFB. Database link P49765
Carrier free

Yes

Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab233505 purified by affinity chromatography and then biotinylated.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VEGFB also known as vascular endothelial growth factor B is a member of the VEGF family. It has a molecular mass of approximately 34-35 kDa. VEGFB interacts with its receptors to promote angiogenesis increasing blood supply to tissues. Expression of VEGFB occurs in the heart skeletal muscle and various endothelial cells. It also shows expression in tissues undergoing high metabolic activities. Besides its angiogenic role VEGFB exhibits participation in metabolic regulation.

Biological function summary

VEGFB supports angiogenesis by promoting endothelial cell growth and migration. It acts as a signal protein not forming part of a larger complex. VEGFB binds to VEGFR-1 (Flt-1) receptor which is involved in transmitting the growth-promoting signals. Its function extends to the regulation of fatty acid uptake into tissues highlighting its role in lipid metabolism and energy utilization.

Pathways

The signalling activities of VEGFB are significant in angiogenesis and metabolic pathways. VEGFB interacts closely with the VEGF-A pathway which is vital for forming new blood vessels. It also shares interactive roles with similar proteins such as VEGF-C and VEGF-D contributing to various angiogenic processes and cellular growth signalling systems.

Associated diseases and disorders

Understanding VEGFB's role is important in conditions like cardiovascular disease and metabolic syndrome. Altered VEGFB signalling may link to heart diseases due to its regulatory function in maintaining heart vasculature. VEGFB has connections with VEGFR-1 and VEGF-A indicating its potential involvement in endothelial dysfunction and highlighting its significance in therapeutic strategies targeting such diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Biotin Anti-VEGFB antibody (ab233505), expandable thumbnail

    Western blot - Biotin Anti-VEGFB antibody (ab233505)

    Reduced.

    Lanes 1, 10, 2, 3, 4, 5, 6, 7, 8 and 9: Western blot - Biotin Anti-VEGFB antibody (ab233505) at 0.2 µg/mL

    Lane 11: Western blot - Biotin Anti-VEGFB antibody (ab233505) at 0.2 µg

    Lane 1: Recombinant human VEGFB protein, 250 ng

    Lane 2: Recombinant human VEGFB protein, 125 ng

    Lane 3: Recombinant human VEGFB protein, 62.5 ng

    Lane 4: Recombinant human VEGFB protein, 31.25 ng

    Lane 5: Recombinant human VEGFB protein, 15.625 ng

    Lane 6: Recombinant human VEGFB protein, 7.8 ng

    Lane 7: Recombinant human VEGFB protein, 3.9 ng

    Lane 8: Recombinant human VEGFB protein, 1.95 ng

    Lane 9: Recombinant human VEGFB protein, 0.98 ng

    Lane 10: Recombinant human VEGFB protein, 0.49 ng

    Lane 11: Recombinant human VEGFB protein, 0.24 ng

    Predicted band size: 22 kDa

  • Western blot - Biotin Anti-VEGFB antibody (ab233505), expandable thumbnail

    Western blot - Biotin Anti-VEGFB antibody (ab233505)

    Non-reduced.

    All lanes: Western blot - Biotin Anti-VEGFB antibody (ab233505) at 0.2 µg/mL

    Lane 1: Recombinant human VEGFB protein, 250 ng

    Lane 2: Recombinant human VEGFB protein, 125 ng

    Lane 3: Recombinant human VEGFB protein, 62.5 ng

    Lane 4: Recombinant human VEGFB protein, 31.25 ng

    Lane 5: Recombinant human VEGFB protein, 15.625 ng

    Lane 6: Recombinant human VEGFB protein, 7.8 ng

    Lane 7: Recombinant human VEGFB protein, 3.9 ng

    Lane 8: Recombinant human VEGFB protein, 1.95 ng

    Lane 9: Recombinant human VEGFB protein, 0.98 ng

    Lane 10: Recombinant human VEGFB protein, 0.49 ng

    Lane 11: Recombinant human VEGFB protein, 0.24 ng

    Predicted band size: 22 kDa

  • Sandwich ELISA - Biotin Anti-VEGFB antibody (ab233505), expandable thumbnail

    Sandwich ELISA - Biotin Anti-VEGFB antibody (ab233505)

    ab233505, in conjunction with Anti-human VEGFB as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant human VEGFB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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