Rabbit Recombinant Monoclonal VEGFD antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-VEGFD antibody [EPR8457] (AB204679), expandable thumbnail](https://content.abcam.com/products/images/biotin-vegfd-antibody-epr8457-ab204679--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123530.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-VEGFD antibody [EPR8457] (AB204679)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
2 products for Alternative Version
Target data
Function
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Alternative names
FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor
Recommended products
Rabbit Recombinant Monoclonal VEGFD antibody - conjugated to Biotin. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Biotin
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8457
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGFD also known as vascular endothelial growth factor D plays a role in angiogenesis and lymphangiogenesis by interacting with VEGF receptors. This protein has a molecular mass of approximately 32 kDa. It is typically expressed in the lungs heart skeletal muscle and small intestine. VEGFD undergoes several proteolytic processes to gain full activity highlighting its functional complexity in the body.
- Biological function summary
Vascular endothelial growth factor D promotes the proliferation and migration of endothelial cells facilitating vascular formation and remodeling. It forms part of the VEGF protein family which is known for its involvement in various vascular processes. The protein's action is largely mediated through its binding to VEGFR-2 and VEGFR-3. This binding activates downstream signaling pathways that contribute to the growth and maintenance of blood and lymphatic vessels.
- Pathways
The functioning of VEGFD is important in the vascular endothelial growth factor signaling pathway and the PI3K-Akt signaling pathway. These pathways are essential for the regulation of cellular processes such as survival growth and motility of endothelial cells. VEGFD is closely related to other members of the VEGF family including VEGFA and VEGFC which similarly interact with VEGF receptors to regulate vascular development and function.
- Associated diseases and disorders
Abnormal levels or activity of VEGFD can lead to pathological conditions such as cancer and lymphedema. In several cancers VEGFD is implicated in tumor angiogenesis aiding the tumor in acquiring nutrients and oxygen. Furthermore in lymphedema issues related to lymphatic vessel development can involve VEGFD alongside VEGFC contributing to fluid retention and tissue swelling. These associations highlight the importance of understanding VEGFD's functions in various pathological contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-VEGFD antibody [EPR8457] (ab204679), expandable thumbnail](https://content.abcam.com/products/images/biotin-vegfd-antibody-epr8457-ab204679--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123530.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-VEGFD antibody [EPR8457] (ab204679)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-VEGFD antibody [EPR8457] (ab204679)
IHC image of VEGFD staining in a section of formalin-fixed paraffin-embedded human heart muscle*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, performed on a Leica BONDTM. The section was then incubated overnight at +4°C with ab204679 at 1/100 dilution. Detection was via an HRP conjugated ABC system and DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Downloads
Product protocols
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