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AB324658

Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3]

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Mouse Recombinant Monoclonal BIRA antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Escherichia coli K-12, Transfected cell line - Escherichia coli K-12 samples.

View Alternative Names

bioR, dhbB, b3973, JW3941, birA, Bifunctional ligase/repressor BirA, Biotin operon repressor, Biotin--[acetyl-CoA-carboxylase] ligase, Biotin--protein ligase, Biotin-[acetyl-CoA carboxylase] synthetase

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] (AB324658)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] (AB324658)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling BirA/TurboID/miniTurbo with ab324658 at 1/500 (2.2 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with an E. coli birA and E. coli TurboID and E. coli miniTurbo expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at (1/1000) 2 ug/ml dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at (1/1000) 2 ug/ml dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at (1/1000) 2 ug/ml dilution.

Western blot - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] (AB324658)
  • WB

Supplier Data

Western blot - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] (AB324658)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] (ab324658) at 1/5000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his-tag whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a E. coli birA expression vector containing a myc-his-tag at 4 µg

Lane 3:

293T cells transfected with a E. coli TurboID expression vector containing a myc-his-tag at 4 µg

Lane 4:

293T cells transfected with a E. coli miniTurbo expression vector containing a myc-his-tag at 4 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 10s

  • Carrier free

    Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5B11c3-3

Isotype

IgG1

Carrier free

No

Reacts with

Escherichia coli K-12

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli K-12": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Escherichia coli K-12": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Escherichia coli K-12": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
See full target information birA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com