Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] - BSA and Azide free (AB324659)

This data was developed using ab324658, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling BirA/TurboID/miniTurbo with ab324658 at 1/500 (2.2 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with an E. coli birA and E. coli TurboID and E. coli miniTurbo expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at (1/1000) 2 ug/ml dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at (1/1000) 2 ug/ml dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at (1/1000) 2 ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] - BSA and Azide free (AB324659)

This data was developed using ab324658, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-BirA/TurboID/miniTurbo antibody [5B11c3-3] (ab324658) at 1/5000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his-tag whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a E. coli birA expression vector containing a myc-his-tag at 4 µg

Lane 3:

293T cells transfected with a E. coli TurboID expression vector containing a myc-his-tag at 4 µg

Lane 4:

293T cells transfected with a E. coli miniTurbo expression vector containing a myc-his-tag at 4 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 10s