AB19609

Anti-BIRC6/APOLLON antibody

Name: Anti-BIRC6/APOLLON antibody (ab19609) | Abcam
Brand: Abcam Limited
SKU: AB19609
Price: 450 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(14 Publications)

Rabbit Polyclonal BIRC6/APOLLON antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human BIRC6 aa 4750-4850.

View Alternative Names

KIAA1289, BIRC6, Dual E2 ubiquitin-conjugating enzyme/E3 ubiquitin-protein ligase BIRC6, BIR repeat-containing ubiquitin-conjugating enzyme, Baculoviral IAP repeat-containing protein 6, Ubiquitin-conjugating BIR domain enzyme apollon, BRUCE, APOLLON

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody (AB19609)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody (AB19609)

Immunohistochemistry analysis of formalin-fixed paraffin-embedded human breast carcinoma tissue staining BIRC6/Apollon with ab19609 at 0.2μg/ml

Supplier Data

Immunoprecipitation - Anti-BIRC6/APOLLON antibody (AB19609)

Immunoprecipitaction of HeLa cells with ab19609

Lane 1 : ab19609 current lot

Lane 2 : ab19609 previous lot

Lane 3 : control IgG

All lanes:

Immunoprecipitation - Anti-BIRC6/APOLLON antibody (ab19609)

Predicted band size: 530 kDa

false

Supplier Data

Western blot - Anti-BIRC6/APOLLON antibody (AB19609)

All lanes:

Western blot - Anti-BIRC6/APOLLON antibody (ab19609) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK293T whole cell lysate at 50 µg

Lane 4:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 530 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human BIRC6 aa 4750-4850. The exact immunogen used to generate this antibody is proprietary information.

Q9NR09

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Anti-apoptotic protein known as inhibitor of apoptosis (IAP) which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase (PubMed : 14765125, PubMed : 15200957, PubMed : 18329369). Unlike most IAPs, does not contain a RING domain and it is not a RING-type E3 ligase (PubMed : 15200957, PubMed : 36758104, PubMed : 36758105, PubMed : 36758106). Instead acts as a dual E2/E3 enzyme that combines ubiquitin conjugating (E2) and ubiquitin ligase (E3) activities in a single polypeptide (PubMed : 15200957, PubMed : 36758104, PubMed : 36758105, PubMed : 36758106). Ubiquitination is mediated by a non-canonical E1 ubiquitin activating enzyme UBA6 (PubMed : 36758104, PubMed : 36758105, PubMed : 36758106). Ubiquitinates CASP3, CASP7 and CASP9 and inhibits their caspase activity; also ubiquitinates their procaspases but to a weaker extent (PubMed : 15200957, PubMed : 36758104, PubMed : 36758105, PubMed : 36758106). Ubiquitinates pro-apoptotic factors DIABLO/SMAC and HTRA2 (PubMed : 15200957, PubMed : 36758104, PubMed : 36758105, PubMed : 36758106). DIABLO/SMAC antagonizes the caspase inhibition activity of BIRC6 by competing for the same binding sites as the caspases (PubMed : 18329369, PubMed : 36758106). Ubiquitinates the autophagy protein MAP1LC3B; this activity is also inhibited by DIABLO/SMAC (PubMed : 36758105). Important regulator for the final stages of cytokinesis (PubMed : 18329369). Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification (PubMed : 18329369).

See full target information BIRC6

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115087 PubMed39705142

2024

The differential interactomes of the KRAS splice variants identify BIRC6 as a ubiquitin ligase for KRAS4A.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Kochen Rossi,Cristina Nuevo-Tapioles,Rachel A O'Keefe,Moritz Hunkeler,Anna M Schmoker,Mercedes Fissore-O'Leary,Wenjuan Su,Ian M Ahearn,Cristina Branco,Hakyung Cheong,Dominic Esposito,Ioana Clotea,Beatrix Ueberheide,Eric S Fischer,Mark R Philips

Diagnostics (Basel, Switzerland) 13: PubMed38066801

2023

Evaluation of BIRC6 Expression in Oral Squamous Cell Carcinoma, Epithelial Dysplasia, Lichen Planus with and without Dysplasia, and Hyperkeratosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fateme Eskandari,Alireza Razavian,Razieh Zare,Shayan Ejlali,Alireza Razmahang,Milad Zanjani,Seyedeh Sara Aghili,Mohammad Amin Mahdiyar,Hossein Mofidi,Kamyar Abbasi,Ashkan Badkoobeh,Nafiseh Shamloo,Lotfollah Kamali Hakim,Ahmed Hussain,Hamid Tebyaniyan

Frontiers in genetics 13:834935 PubMed36105077

2022

Identification of an autophagy-related 12-lncRNA signature and evaluation of NFYC-AS1 as a pro-cancer factor in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Tong,Lifa Xu,Sheng Xu,Mingming Zhang

Frontiers in oncology 12:895106 PubMed35860596

2022

Cold Atmospheric Plasma Conveys Selectivity Against Hepatocellular Carcinoma Cells Triggering EGFR(Tyr1068)-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Danjun Wang,Jianying Zhang,Linhan Cai,Xiaofeng Dai

Molecular therapy. Nucleic acids 26:798-812 PubMed34729249

2021

Overexpression of BIRC6 driven by EGF-JNK-HECTD1 signaling is a potential therapeutic target for triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongpeng Li,Yanan Tan,Lijuan Wen,Zhihao Xing,Changxu Wang,Liuhui Zhang,Kai Wu,Haiyan Sun,Yuqing Li,Qifang Lei,Song Wu

Small (Weinheim an der Bergstrasse, Germany) 16:e1907686 PubMed32227449

2020

Next-Generation Sequencing Reveals Differential Responses to Acute versus Long-Term Exposures to Graphene Oxide in Human Lung Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sourav P Mukherjee,Govind Gupta,Katharina Klöditz,Jun Wang,Artur Filipe Rodrigues,Kostas Kostarelos,Bengt Fadeel

Biomedicine & pharmacotherapy = Biomedecine & phar 112:108679 PubMed30798133

2019

Alpha-lipoic acid attenuates p-cresyl sulfate-induced renal tubular injury through suppression of apoptosis and autophagy in human proximal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Sun Park,Hoon In Choi,Dong-Hyun Kim,Chang Seong Kim,Eun Hui Bae,Seong Kwon Ma,Soo Wan Kim

PloS one 12:e0177871 PubMed28520795

2017

BIRC6 mediates imatinib resistance independently of Mcl-1.

Applications

Species

Human

Denis O Okumu,Michael P East,Merlin Levine,Laura E Herring,Raymond Zhang,Thomas S K Gilbert,David W Litchfield,Yanping Zhang,Lee M Graves

Oncology letters 12:5129-5135 PubMed28105219

2016

Expression and clinical significance of Apollon in renal carcinoma.

Applications

WB, IHC

Species

Unspecified reactive species, Unspecified reactive species

Hongbo Guo,Weifeng Zhong,Xiaohong Wang,Bin Pan,Feng Li,Kuang Lu,Zexuan Su,Shiqing Zhang

Molecular medicine reports 14:1933-40 PubMed27432467

2016

Expression and clinical significance of Apollon in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Bo-Lin Chen,Yan-Wu Zhou,Ren-Wei Guo,Meng-Ting Shuai,Jun-Xian Zeng,Ai-Min Leng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BIRC6/APOLLON antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody (AB19609)

Immunoprecipitation - Anti-BIRC6/APOLLON antibody (AB19609)

Western blot - Anti-BIRC6/APOLLON antibody (AB19609)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise