Anti-BIRC6/APOLLON antibody [EPR24925-145]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BIRC6 with ab291072 at 1/500 (1.218 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat testis. The section was incubated with ab291072 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072)

Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling BIRC6 with ab291072 at 1/500 (1.218 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse liver cancer. The section was incubated with ab291072 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BIRC6 with ab291072 at 1/500 (1.218 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse testis. The section was incubated with ab291072 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 µg

Lane 3:

A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 530 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression level of BIRC6 protein is downregulated in response to doxorubicin treatment (PMID : 23409057). Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

Lane 2:

HeLa treated with /mL doxorubicin for 24 hours, whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 530 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/2000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 530 kDa

false

Exposure time: 48s