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Rabbit Recombinant Monoclonal BIRC6/APOLLON antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072), expandable thumbnail
  • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072), expandable thumbnail
  • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (AB291072), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/2000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Anti-apoptotic protein known as inhibitor of apoptosis (IAP) which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase (PubMed:14765125, PubMed:15200957, PubMed:18329369). Unlike most IAPs, does not contain a RING domain and it is not a RING-type E3 ligase (PubMed:15200957, PubMed:36758104, PubMed:36758105, PubMed:36758106). Instead acts as a dual E2/E3 enzyme that combines ubiquitin conjugating (E2) and ubiquitin ligase (E3) activities in a single polypeptide (PubMed:15200957, PubMed:36758104, PubMed:36758105, PubMed:36758106). Ubiquitination is mediated by a non-canonical E1 ubiquitin activating enzyme UBA6 (PubMed:36758104, PubMed:36758105, PubMed:36758106). Ubiquitinates CASP3, CASP7 and CASP9 and inhibits their caspase activity; also ubiquitinates their procaspases but to a weaker extent (PubMed:15200957, PubMed:36758104, PubMed:36758105, PubMed:36758106). Ubiquitinates pro-apoptotic factors DIABLO/SMAC and HTRA2 (PubMed:15200957, PubMed:36758104, PubMed:36758105, PubMed:36758106). DIABLO/SMAC antagonizes the caspase inhibition activity of BIRC6 by competing for the same binding sites as the caspases (PubMed:18329369, PubMed:36758106). Ubiquitinates the autophagy protein MAP1LC3B; this activity is also inhibited by DIABLO/SMAC (PubMed:36758105). Important regulator for the final stages of cytokinesis (PubMed:18329369). Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification (PubMed:18329369).

Alternative names

KIAA1289, BIRC6, Dual E2 ubiquitin-conjugating enzyme/E3 ubiquitin-protein ligase BIRC6, BIR repeat-containing ubiquitin-conjugating enzyme, Baculoviral IAP repeat-containing protein 6, Ubiquitin-conjugating BIR domain enzyme apollon, BRUCE, APOLLON

Recommended products

Rabbit Recombinant Monoclonal BIRC6/APOLLON antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24925-145
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072)

    Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling BIRC6 with ab291072 at 1/500 (1.218 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse liver cancer. The section was incubated with ab291072 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BIRC6 with ab291072 at 1/500 (1.218 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat testis. The section was incubated with ab291072 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BIRC6 with ab291072 at 1/500 (1.218 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse testis. The section was incubated with ab291072 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072), expandable thumbnail

    Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/2000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 530 kDa

    Exposure time: 48s

  • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072), expandable thumbnail

    Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression level of BIRC6 protein is downregulated in response to doxorubicin treatment (PMID: 23409057).
    Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

    Lane 2: HeLa treated with /mL doxorubicin for 24 hours, whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 530 kDa

    Exposure time: 81s

  • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072), expandable thumbnail

    Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 µg

    Lane 3: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 530 kDa

    Exposure time: 48s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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