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AB70525

Anti-BLAP75 antibody

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(4 Publications)

Rabbit Polyclonal BLAP75 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RMI1 aa 250-300.

View Alternative Names

C9orf76, RMI1, RecQ-mediated genome instability protein 1, BLM-associated protein of 75 kDa, FAAP75, BLAP75

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLAP75 antibody (AB70525)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLAP75 antibody (AB70525)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BLAP75 with ab70525 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-BLAP75 antibody (AB70525)
  • IP

Unknown

Immunoprecipitation - Anti-BLAP75 antibody (AB70525)

Detection of Human BLAP75 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70525 at 3 µg/mg for IP and at 1 µg/ml for subsequent WB detection.

All lanes:

Immunoprecipitation - Anti-BLAP75 antibody (ab70525)

Predicted band size: 70 kDa

false

Western blot - Anti-BLAP75 antibody (AB70525)
  • WB

Unknown

Western blot - Anti-BLAP75 antibody (AB70525)

All lanes:

Western blot - Anti-BLAP75 antibody (ab70525) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Predicted band size: 70 kDa

Observed band size: 110 kDa,170 kDa,190 kDa,238 kDa,32 kDa,60 kDa,74 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human RMI1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q9H9A7

Specificity

We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
See full target information RMI1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed39704103

2024

The interplay of the translocase activity and protein recruitment function of PICH in ultrafine anaphase bridge resolution and genomic stability.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Kong,Kun Chen,Primrose Chanboonyasitt,Huadong Jiang,Ka Yan Wong,Hoi Tang Ma,Ying Wai Chan

The EMBO journal 40:e106355 PubMed34128550

2021

PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel González-Acosta,Elena Blanco-Romero,Patricia Ubieto-Capella,Karun Mutreja,Samuel Míguez,Susana Llanos,Fernando García,Javier Muñoz,Luis Blanco,Massimo Lopes,Juan Méndez

Experimental and therapeutic medicine 14:2189-2193 PubMed28962140

2017

Relationship between blood lactic acid, blood procalcitonin, C-reactive protein and neonatal sepsis and corresponding prognostic significance in sick children.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfeng Jia,Ying Wang,Xinhua Yu

The EMBO journal 30:3309-21 PubMed21743438

2011

PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution.

Applications

WB

Species

Human

Yuwen Ke,Jae-Wan Huh,Ross Warrington,Bing Li,Nan Wu,Mei Leng,Junmei Zhang,Haydn L Ball,Bing Li,Hongtao Yu
View all publications
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Product promise

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