Rabbit Polyclonal BLAP75 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RMI1 aa 250-300.
Images
Publications
Filter by
- The EMBO journal 40:e1063552021PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel González-Acosta,Elena Blanco-Romero,Patricia Ubieto-Capella,Karun Mutreja,Samuel Míguez,Susana Llanos,Fernando García,Javier Muñoz,Luis Blanco,Massimo Lopes,Juan MéndezPubMed 34128550
- Experimental and therapeutic medicine 14:2189-21932017Relationship between blood lactic acid, blood procalcitonin, C-reactive protein and neonatal sepsis and corresponding prognostic significance in sick children.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongfeng Jia et. al.PubMed 28962140
- The EMBO journal 30:3309-212011PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RMI1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9A7
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Target data
Function
Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
Alternative names
C9orf76, RMI1, RecQ-mediated genome instability protein 1, BLM-associated protein of 75 kDa, FAAP75, BLAP75
Recommended products
Rabbit Polyclonal BLAP75 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RMI1 aa 250-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RMI1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9A7
- Purification technique
- Affinity purification Immunogen
- Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-BLAP75 antibody (ab70525)
All lanes: Western blot - Anti-BLAP75 antibody (ab70525) at 0.04 µg/mL
Lane 1: Whole cell lysate from HeLa cells at 50 µg
Lane 2: Whole cell lysate from HeLa cells at 15 µg
Lane 3: Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Observed band size: 110 kDa, 170 kDa, 190 kDa, 238 kDa, 32 kDa, 60 kDa, 74 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLAP75 antibody (ab70525)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BLAP75 with ab70525 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-BLAP75 antibody (ab70525)
Detection of Human BLAP75 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70525 at 3 µg/mg for IP and at 1 µg/ml for subsequent WB detection.
All lanes: Immunoprecipitation - Anti-BLAP75 antibody (ab70525)
Predicted band size: 70 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com