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AB110099

Anti-BLBP antibody

5

(2 Reviews)

|

(5 Publications)

Goat Polyclonal BLBP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FABP7 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BLBP, FABPB, MRG, FABP7, Brain lipid-binding protein, Brain-type fatty acid-binding protein, Fatty acid-binding protein 7, Mammary-derived growth inhibitor related, B-FABP

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)

ab110099 at 2.5μg/ml staining BLBP in formalin-fixed, paraffin-embedded Human heart tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)

ab110099 at 2.5μg/ml staining BLBP in formalin-fixed, paraffin-embedded Human skeletal muscle tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)

ab110099 at 2.5μg/ml staining BLBP in formalin-fixed, paraffin-embedded Human cerebellum tissue after heat induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, the slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)
  • IHC-P

AbReview45047****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody (AB110099)

ab110099 staining BLBP in Xenopus laevis gindbrain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution, permeabilized with 0.4% Triton X-100 in 3% normal horse serum and blocked with 3% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in 10mM citrate buffer. Samples were incubated with primary antibody (1/100 in 0.4% Triton X-100 in 3% normal horse serum) for 12 hours at 4°C. An undiluted HRP-conjugated horse anti-goat IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Bharti Katbamna

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human FABP7 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O15540

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).
See full target information FABP7

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 8:31 PubMed37328477

2023

A short dasatinib and quercetin treatment is sufficient to reinstate potent adult neuroregenesis in the aged killifish.

Applications

Unspecified application

Species

Unspecified reactive species

Jolien Van Houcke,Valerie Mariën,Caroline Zandecki,Rajagopal Ayana,Elise Pepermans,Kurt Boonen,Eve Seuntjens,Geert Baggerman,Lutgarde Arckens

Laboratory investigation; a journal of technical methods and pathology 103:100179 PubMed37224922

2023

Impact of P-selectin-PSGL-1 Axis on Platelet-Endothelium-Leukocyte Interactions in Fatal COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Massimo Granai,Verena Warm,Antonio Vogelsberg,Jakob Milla,Karen Greif,Ulrich Vogel,Tamam Bakchoul,Peter Rosenberger,Leticia Quintanilla-Martinez,Christian M Schürch,Karin Klingel,Falko Fend,Hans Bösmüller

Current research in neurobiology 3:100042 PubMed36518338

2022

Plasticity of cell proliferation in the retina of fish under light and darkness conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Inés Berrosteguieta,Juan Carlos Rosillo,María Laura Herrera,Silvia Olivera-Bravo,Gabriela Casanova,Vicente Herranz-Pérez,José Manuel García-Verdugo,Anabel Sonia Fernández

Aging cell 20:e13464 PubMed34428340

2021

Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri.

Applications

Unspecified application

Species

Unspecified reactive species

Jolien Van Houcke,Valerie Mariën,Caroline Zandecki,Sophie Vanhunsel,Lieve Moons,Rajagopal Ayana,Eve Seuntjens,Lutgarde Arckens

Frontiers in cell and developmental biology 7:303 PubMed31850342

2019

Human Cerebral Organoids and Fetal Brain Tissue Share Proteomic Similarities.

Applications

Unspecified application

Species

Unspecified reactive species

Juliana Minardi Nascimento,Verônica M Saia-Cereda,Rafaela C Sartore,Rodrigo Madeiro da Costa,Clarissa S Schitine,Hercules Rezende Freitas,Michael Murgu,Ricardo A de Melo Reis,Stevens K Rehen,Daniel Martins-de-Souza
View all publications

Product promise

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