Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)

Rabbit Recombinant Monoclonal BLBP antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (AB279652), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-Fr	Flow Cyt (Intra)	IHC-P
Human	Not recommended	Not recommended	Tested	Expected	Expected	Tested
Mouse	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Tested	Expected	Tested	Not recommended	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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1 product for Alternative Version

Target data

See full target information FABP7

Function

B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).

Alternative names

BLBP, FABPB, MRG, FABP7, Brain lipid-binding protein, Brain-type fatty acid-binding protein, Fatty acid-binding protein 7, Mammary-derived growth inhibitor related, B-FABP

Recommended products

Rabbit Recombinant Monoclonal BLBP antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR24033-13
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab279652 is the carrier-free version of Anti-BLBP antibody [EPR24033-13] ab279649.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Western blot - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, kidney (PMID: 16618771)
Exposure time: 10 seconds
All lanes: Western blot - Anti-BLBP antibody [EPR24033-13] (Anti-BLBP antibody [EPR24033-13] ab279649) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human spleen tissue lysate at 20 µg
Lane 3: Human kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum (PMID: 16623952). The section was incubated with Anti-BLBP antibody [EPR24033-13] ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
BLBP was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10 μg with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-BLBP antibody [EPR24033-13] ab279649 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10 μg
Lane 2: Anti-BLBP antibody [EPR24033-13] ab279649 IP in mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-BLBP antibody [EPR24033-13] ab279649 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-BLBP antibody [EPR24033-13] (Anti-BLBP antibody [EPR24033-13] ab279649)
Predicted band size: 14 kDa
Observed band size: 15 kDa
Immunohistochemistry (Frozen sections) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum, which partially co-stained with GFAP (Red) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary gliocyte. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: Anti-BLBP antibody [EPR24033-13] ab279649 at a 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.
Negative control 2: Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at a 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized mouse primary neural/glia cells labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/500 dilution (Right panel) compared with Rabbit monoclonal IgG Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor^® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human astrocytoma labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma (PMID: 16623952). The section was incubated with Anti-BLBP antibody [EPR24033-13] ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with Anti-BLBP antibody [EPR24033-13] ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on human kidney (PMID: 9375786).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, kidney (PMID: 16618771)
Exposure time: 6 seconds
All lanes: Western blot - Anti-BLBP antibody [EPR24033-13] (Anti-BLBP antibody [EPR24033-13] ab279649) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with Anti-BLBP antibody [EPR24033-13] ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with Anti-BLBP antibody [EPR24033-13] ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Negative control: no staining on mouse kidney.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times:
Lane 1: 3 seconds
Lane 2: 26 seconds
All lanes: Western blot - Anti-BLBP antibody [EPR24033-13] (Anti-BLBP antibody [EPR24033-13] ab279649) at 1/1000 dilution
Lane 1: Rat brain cortex tissue lysate at 20 µg
Lane 2: Rat cerebellum tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/2000 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with Anti-BLBP antibody [EPR24033-13] ab279649 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labelling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary gliocyte. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: Anti-BLBP antibody [EPR24033-13] ab279649 at a 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.
Negative control 2: Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at a 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-BLBP antibody [EPR24033-13] - BSA and Azide free (ab279652)
This data was developed using Anti-BLBP antibody [EPR24033-13] ab279649, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized rat primary neural/glia cells labeling BLBP with Anti-BLBP antibody [EPR24033-13] ab279649 at 1/500 dilution (Right panel) compared with Rabbit monoclonal IgG Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/2000 dilution was used as the secondary antibody.