AB2230

Anti-BLNK antibody

Name: Anti-BLNK antibody (ab2230) | Abcam
Brand: Abcam Limited
SKU: AB2230
Price: 535 USD
Availability: InStock

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(1 Publication)

Goat Polyclonal BLNK antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BLNK aa 400 to C-terminus.

View Alternative Names

BASH, SLP65, BLNK, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65

2 Images

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Western blot - Anti-BLNK antibody (AB2230)

ab2230 staining (4μg/ml) of Daudi lysate (RIPA buffer, 30μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour.

All lanes:

Western blot - Anti-BLNK antibody (ab2230) at 4 µg/mL

All lanes:

Cell lysate prepared from human Daudi cells at 35 µg

Predicted band size: 50 kDa

Observed band size: 70 kDa

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Western blot - Anti-BLNK antibody (AB2230)

All lanes:

Western blot - Anti-BLNK antibody (ab2230) at 1 µg/mL

Lane 1:

Cell lysate prepared from HEK293 mock transfected cells

Lane 2:

Cell lysate prepared from HEK293 overexpressing BLNK

Predicted band size: 50 kDa

Observed band size: 50 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human BLNK aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WV28

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.05% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

See full target information BLNK

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communications 355:745-50 PubMed17320047

2007

PACSIN3 overexpression increases adipocyte glucose transport through GLUT1.

Applications

Unspecified application

Species

Unspecified reactive species

William Roach,Markus Plomann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BLNK antibody

Western blot - Anti-BLNK antibody (AB2230)

Western blot - Anti-BLNK antibody (AB2230)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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