Rabbit Monoclonal BLNK phospho Y84 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] (AB174837), expandable thumbnail](https://content.abcam.com/products/images/blnk-phospho-y84-antibody-ep22022-ab174837--western-blot-img109131.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] (AB174837)")
Publications
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- World journal of gastroenterology 28:5865-58802022Curcumin alleviates experimental colitis a potential mechanism involving memory B cells and Bcl-6-Syk-BLNK signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi-Yi Wei,Tian-Tian Wu,Jia-Qi Huang,Zeng-Ping Kang,Meng-Xue Wang,You-Bao Zhong,Wei Ge,Bu-Gao Zhou,Hai-Mei Zhao,Hai-Yan Wang,Duan-Yong LiuPubMed 36353208
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
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5 products for Alternative Product
Target data
Function
Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Alternative names
BASH, SLP65, BLNK, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65
Recommended products
Rabbit Monoclonal BLNK phospho Y84 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP2202(2)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] (ab174837), expandable thumbnail](https://content.abcam.com/products/images/blnk-phospho-y84-antibody-ep22022-ab174837--western-blot-img109131.jpg "Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] (ab174837)")
Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] (ab174837)
All lanes: Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] (ab174837) at 1/1000 dilution
Lane 1: Raji cell lysate, untreated at 10 µg
Lane 2: Raji cell lysate, treated with pervanadate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
Downloads
Product protocols
- Visit the general protocols
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