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AB284825

Anti-BLNK (phospho Y84) antibody [EP2202(2)] - BSA and Azide free

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Rabbit Monoclonal BLNK phospho Y84 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

BASH, SLP65, BLNK, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65

2 Images
Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] - BSA and Azide free (AB284825)
  • WB

Supplier Data

Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] - BSA and Azide free (AB284825)

This data was developed using ab174837, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] (<a href='/en-us/products/primary-antibodies/blnk-phospho-y84-antibody-ep22022-ab174837'>ab174837</a>) at 1/1000 dilution

Lane 1:

Raji cell lysate, untreated at 10 µg

Lane 2:

Raji cell lysate, treated with pervanadate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 50 kDa

true

Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] - BSA and Azide free (AB284825)
  • WB

Supplier Data

Western blot - Anti-BLNK (phospho Y84) antibody [EP2202(2)] - BSA and Azide free (AB284825)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The extra bands have not been defined.

Lane 1:

Untreated Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Lane 2:

Raji treated with 1mM pervanadate for 30min whole cell lysate at 15 µg

Lane 3:

Raji treated with 1mM pervanadate for 30min whole cell lysate, then the membrane was incubated with phosphatase at 15 µg

Secondary

Lanes 1 - 3:

Goat Anti-Rabbit IgG (HRP) at 1/2000 dilution

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 7s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2202(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284825 is the carrier-free version of ab174837.

This product has switched from a hybridoma to recombinant production method on 20th November 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
See full target information BLNK phospho Y84
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com