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AB2524

Anti-Blood Group B Antigen antibody [HEB-29]

3

(1 Review)

|

(8 Publications)

Mouse Monoclonal ABO antibody. Carrier free. Suitable for Agg, IHC-P and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase, GTA, B transferase, GTB, ABO

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HEB-29

Isotype

IgM

Light chain type

unknown

Carrier free

Yes

Reacts with

Human

Applications

Agg, IHC-P

applications

Specificity

This antibody recognizes human blood group B antigens. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood.

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Alternative Products

Primary Antibodies

AB24224

Anti-Blood Group B Antigen antibody [Z5H-2]

  • 0
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Complementary Products

Assay Kits

AB270207

Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase)

Recombinant|SimpleStep

Sandwich ELISA - Human ABO ELISA Kit (Histo-Blood Group ABO System Transferase) (AB270207)

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Primary Antibodies

AB2521

Anti-Blood Group A Antigen antibody [HE-193]

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  • 0
Primary Antibodies

AB24224

Anti-Blood Group B Antigen antibody [Z5H-2]

  • 0
  • 0
Primary Antibodies

AB20131

Anti-Blood Group A Antigen antibody [9A]

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Agg" : {"fullname" : "Agglutination", "shortname":"Agg"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Agg-species-checked": "guaranteed", "Agg-species-dilution-info": "", "Agg-species-notes": "<p>The minimum titre is 1:128 with erythrocytes of blood group B.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Concentrated by ultrafiltration (100 kDa cut-off). Actual immunoglobulin concentration not determined.
Storage buffer
Constituents: Tissue culture supernatant
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Blood Group B Antigen commonly called the B antigen is a carbohydrate antigen found on the surface of red blood cells. This antigen is part of the ABO blood group system which determines an individual's blood type. The B antigen's molecular composition consists primarily of a sugar molecule called D-galactose. The B antigen is expressed on the erythrocyte surface and is encoded by the B allele of the ABO gene. Individuals with blood type B will have the B antigen on the surface of their red blood cells and do not have anti-B antibodies in their plasma. The mass of the B antigen can vary depending on the glycoproteins and glycolipids it attaches to on the membrane.
Biological function summary

The B antigen's presence on red blood cells plays a fundamental role in immune system recognition and compatibility during blood transfusions. It is not part of a complex but associates directly with the cell membrane structures. The presence of particular carbohydrate sequences such as those of the B antigen allows the immune system to distinguish between self and non-self cells. Known as anti-B antibodies these antibodies exist in individuals who do not express the B antigen specifically those with blood type A or O.

Pathways

Blood Group B Antigen contributes significantly to the ABO blood group system's immunological pathway. This pathway includes biosynthesis and degradation processes involving the glycosyltransferase enzyme responsible for adding sugar residues to the precursor substance to form specific antigens. Glycosyltransferase is related to protein pathways that handle energy metabolism and cellular processes due to its enzymatic role in forming cell surface markers like the B antigen.

The B antigen is linked to transfusion reactions and hemolytic disease of the newborn (HDN). Incompatibility between donor and recipient blood types during transfusions can cause immunological reactions where anti-B antibodies attack the B antigens. These reactions can lead to severe complications. HDN occurs when a mother with type O blood carries a baby with type B blood resulting in anti-B antibodies attacking fetal red blood cells. This emphasizes the clinical significance of the B antigen in blood type compatibility and transfusion medicine.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens : A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of N-acetyl-alpha-D-galactosamine (GalNAc)) or to the B antigen (by addition of galactose (Gal)), whereas O individuals lack such activity and express the H antigen precursor unmodified.. Catalyzes the transfer of GalNAc or Gal in an alpha1,3 linkage to the terminal Gal of all five types of naturally occurring H antigens, forming the antigenic structures of the A and B blood groups.
See full target information Histo-blood group ABO system transferase
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1506 PubMed39929829

2025

Enzymatic conversion of blood group B kidney prevents hyperacute antibody-mediated injuries in ABO-incompatible transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zeng,Ming Ma,Xiaojuan Jiang,Zhengsheng Rao,Dan Huang,Hao Zhang,Saifu Yin,Rong Bao,Haohan Zhang,Zhiling Wang,Hongwei Gao,Feng Gong,Tao Lin,Keqin Zhang,Turun Song

International journal of molecular sciences 22: PubMed34208903

2021

Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Säljö,Anders Thornell,Chunsheng Jin,Peter Stålberg,Olov Norlén,Susann Teneberg

Journal of orthopaedic translation 21:129-135 PubMed32309138

2020

Association between the ABO blood group and primary knee osteoarthritis: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Changchuan Li,Nengtai Ouyang,Xiuju Wang,Anjing Liang,Yingqian Mo,Shixun Li,Junxiong Qiu,Guibin Fang,Yuan Fu,Bin Song,Zhong Chen,Yue Ding

Molecular & cellular proteomics : MCP 16:743-758 PubMed28461410

2017

Structural Diversity of Human Gastric Mucin Glycans.

Applications

Unspecified application

Species

Unspecified reactive species

Chunsheng Jin,Diarmuid T Kenny,Emma C Skoog,Médea Padra,Barbara Adamczyk,Varvara Vitizeva,Anders Thorell,Vignesh Venkatakrishnan,Sara K Lindén,Niclas G Karlsson

Proceedings of the National Academy of Sciences of 112:13057-62 PubMed26438868

2015

Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Tsu Chen,Chein-Hung Chen,Ko-Li Ku,Michael Hsiao,Chun-Pin Chiang,Tsui-Ling Hsu,Min-Huey Chen,Chi-Huey Wong

Glycoconjugate journal 32:393-412 PubMed26104834

2015

Characterization of moose intestinal glycosphingolipids.

Applications

Unspecified application

Species

Unspecified reactive species

Miralda Madar Johansson,Benjamin Dedic,Klara Lundholm,Filip Berner Branzell,Angela Barone,John Benktander,Susann Teneberg

The Journal of biological chemistry 288:10035-50 PubMed23404501

2013

Structural complexity of non-acid glycosphingolipids in human embryonic stem cells grown under feeder-free conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Barone,John Benktander,Jonas Ångström,Anders Aspegren,Petter Björquist,Susann Teneberg,Michael E Breimer

Neoplasma 36:479-88 PubMed2475797

1989

Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities.

Applications

Unspecified application

Species

Unspecified reactive species

J Vanák,D Drímalová,O Smyslová,M Nĕmec,V Viklický,K Wisniewski
View all publications
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Product promise

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