JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB24224

Anti-Blood Group B Antigen antibody [Z5H-2]

Be the first to review this product! Submit a review

|

(4 Publications)

Mouse Monoclonal ABO antibody. Suitable for IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Z5H-2

Isotype

IgM

Carrier free

No

Reacts with

Human

Applications

ELISA, IP, WB, ICC/IF, Flow Cyt

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS
Storage buffer
pH: 7.2 - 7.6 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Blood Group B Antigen commonly called the B antigen is a carbohydrate antigen found on the surface of red blood cells. This antigen is part of the ABO blood group system which determines an individual's blood type. The B antigen's molecular composition consists primarily of a sugar molecule called D-galactose. The B antigen is expressed on the erythrocyte surface and is encoded by the B allele of the ABO gene. Individuals with blood type B will have the B antigen on the surface of their red blood cells and do not have anti-B antibodies in their plasma. The mass of the B antigen can vary depending on the glycoproteins and glycolipids it attaches to on the membrane.
Biological function summary

The B antigen's presence on red blood cells plays a fundamental role in immune system recognition and compatibility during blood transfusions. It is not part of a complex but associates directly with the cell membrane structures. The presence of particular carbohydrate sequences such as those of the B antigen allows the immune system to distinguish between self and non-self cells. Known as anti-B antibodies these antibodies exist in individuals who do not express the B antigen specifically those with blood type A or O.

Pathways

Blood Group B Antigen contributes significantly to the ABO blood group system's immunological pathway. This pathway includes biosynthesis and degradation processes involving the glycosyltransferase enzyme responsible for adding sugar residues to the precursor substance to form specific antigens. Glycosyltransferase is related to protein pathways that handle energy metabolism and cellular processes due to its enzymatic role in forming cell surface markers like the B antigen.

The B antigen is linked to transfusion reactions and hemolytic disease of the newborn (HDN). Incompatibility between donor and recipient blood types during transfusions can cause immunological reactions where anti-B antibodies attack the B antigens. These reactions can lead to severe complications. HDN occurs when a mother with type O blood carries a baby with type B blood resulting in anti-B antibodies attacking fetal red blood cells. This emphasizes the clinical significance of the B antigen in blood type compatibility and transfusion medicine.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Blood Group B Antigen
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 11: PubMed37631871

2023

Maternal and Infant Histo-Blood Group Antigen (HBGA) Profiles and Their Influence on Oral Rotavirus Vaccine (Rotarix) Immunogenicity among Infants in Zambia.

Applications

Unspecified application

Species

Unspecified reactive species

Adriace Chauwa,Samuel Bosomprah,Natasha Makabilo Laban,Bernard Phiri,Mwelwa Chibuye,Obvious Nchimunya Chilyabanyama,Sody Munsaka,Michelo Simuyandi,Innocent Mwape,Cynthia Mubanga,Masuzyo Chirwa Chobe,Caroline Chisenga,Roma Chilengi

Science advances 6:eaaw9679 PubMed32219154

2020

Surface-anchored framework for generating RhD-epitope stealth red blood cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqi Zhao,Mingjie Fan,Yanni Chen,Zhaoming Liu,Changyu Shao,Biao Jin,Xiaoyu Wang,Lanlan Hui,Shuaifei Wang,Zhaoping Liao,Daishun Ling,Ruikang Tang,Ben Wang

BMC research notes 12:607 PubMed31547886

2019

Characterization of human norovirus binding to gut-associated bacterial ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Erin A Almand,Matthew D Moore,Lee-Ann Jaykus

Haematologica 92:1208-15 PubMed17666368

2007

No alloantibodies against mesenchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients.

Applications

ICC/IF

Species

Human

Mikael Sundin,Olle Ringdén,Berit Sundberg,Silvia Nava,Cecilia Götherström,Katarina Le Blanc
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB2524

Anti-Blood Group B Antigen antibody [HEB-29]

  • 3
  • 1

Complementary Products

Primary Antibodies

AB20131

Anti-Blood Group A Antigen antibody [9A]

  • 0
  • 0
Primary Antibodies

AB24213

Anti-Blood Group H ab antigen antibody [97-I]

  • 3
  • 1
Primary Antibodies

AB3967

Anti-Blood Group Lewis a antibody [7LE]

  • 5
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com