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AB231602

Anti-Blood Group Kell Antigen antibody

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(1 Publication)

Rabbit Polyclonal Blood Group Kell Antigen antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Kel aa 1-400.

View Alternative Names

CD238, Kell blood group glycoprotein homolog, Kel

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Kell Antigen antibody (AB231602)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Kell Antigen antibody (AB231602)

Formalin-fixed, paraffin-embedded mouse testis tissue stained for Blood Group Kell Antigen using ab231602 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Blood Group Kell Antigen antibody (AB231602)
  • WB

Supplier Data

Western blot - Anti-Blood Group Kell Antigen antibody (AB231602)

All lanes:

Western blot - Anti-Blood Group Kell Antigen antibody (ab231602) at 2 µg/mL

All lanes:

Mouse spleen lysate

Predicted band size: 83 kDa

false

Western blot - Anti-Blood Group Kell Antigen antibody (AB231602)
  • WB

Supplier Data

Western blot - Anti-Blood Group Kell Antigen antibody (AB231602)

All lanes:

Western blot - Anti-Blood Group Kell Antigen antibody (ab231602) at 2 µg/mL

All lanes:

Mouse testis lysate

Predicted band size: 83 kDa

false

Western blot - Anti-Blood Group Kell Antigen antibody (AB231602)
  • WB

Supplier Data

Western blot - Anti-Blood Group Kell Antigen antibody (AB231602)

All lanes:

Western blot - Anti-Blood Group Kell Antigen antibody (ab231602) at 2 µg/mL

All lanes:

Recombinant mouse Blood Group Kell Antigen protein

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Kel aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q9EQF2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Blood Group Kell Antigen also known as Kell protein is a type II transmembrane glycoprotein with a mass of approximately 93 kDa. It exists on the surface of red blood cells (RBCs) and has enzymatic activity specifically as a zinc endopeptidase. The primary expression site for Kell protein is the erythrocyte membrane where it functions in processing various polypeptides through its enzymatic action. There are different phenotypes like Kell positive and Kell negative and antibodies such as anti-Kell and anti-K are significant in immunohematology.
Biological function summary

The Kell protein facilitates the degradation of endothelin precursors. It belongs to the neprilysin (M13) family of zinc endopeptidases playing a critical part in modulating vasoactive peptides. Normally the Kell protein forms a complex with another protein called XK an integral membrane protein. When properly functioning this Kell-XK complex maintains RBC integrity and membrane flexibility essential for normal cellular operations and lifespan.

Pathways

The Kell antigen influences pathways involved in cardiovascular regulation and cellular movement. It has connections with the vasoregulatory pathway where it interacts with endothelins. The breakdown of endothelin precursors by Kell reduces the levels of these potent vasoconstrictors. The protein also has an indirect association with members of the endothelin-converting enzyme family which further processes these peptides into active forms.

The Kell antigen plays an important role in conditions like transfusion reactions and hemolytic disease of the newborn (HDN). In transfusion medicine incompatibility with Kell positive blood can lead to antibody production resulting in hemolytic reactions. Anti-Kell antibodies such as anti-K and anti-Kell are particularly implicated in HDN where the maternal immune response targets the Kell antigen on fetal erythrocytes. The XK protein related to Kell through the disease mechanism is also involved and mutations in XK can cause McLeod syndrome further complicating blood group antigen relationships.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3 (By similarity).
See full target information Kel
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 598:5427-5451 PubMed32893883

2020

Functional, proteomic and bioinformatic analyses of Nrf2- and Keap1- null skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Lie Gao,Vikas Kumar,Neetha Nanoth Vellichirammal,Song-Young Park,Tara L Rudebush,Li Yu,Won-Mok Son,Elizabeth J Pekas,Ahmed M Wafi,Juan Hong,Peng Xiao,Chittibabu Guda,Han-Jun Wang,Harold D Schultz,Irving H Zucker
View all publications
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