AB3967

Anti-Blood Group Lewis a antibody [7LE]

Name: Anti-Blood Group Lewis a antibody [7LE] (ab3967) | Abcam
Brand: Abcam Limited
SKU: AB3967
Price: 510 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(10 Publications)

Mouse Monoclonal Blood Group Lewis a antibody. Suitable for IHC-P and reacts with Human, Mouse samples. Cited in 10 publications.

View Alternative Names

Lewis A antigen

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7LE

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Blood Group Lewis a also known as Le^a is a carbohydrate antigen expressed on the surface of red blood cells and many epithelial tissues. It is part of the Lewis blood group system and its structure includes fucosylated glycan chains. The mass of the antigen is not readily available due to its nature as a carbohydrate chain component rather than a protein. This antigen can be found on the mucosal surfaces and in secretions where it plays a role in determining the Lewis blood type.

Biological function summary

Blood Group Lewis a serves as a ligand for selectins which are cell adhesion molecules. These interactions are important for cell-cell communication and for immune response modulation. The Lewis a antigen is not a standalone protein and does not function as part of a larger protein complex. Its carbohydrate structure assists in the recognition and binding processes involving selectins during cell signaling.

Pathways

The role of Blood Group Lewis a connects with the glycosylation pathways that modify proteins and lipids by adding sugar moieties. It also interacts with proteins in the cell adhesion pathways like E and P-selectins during the inflammatory response. Fucosyltransferases are the key enzymes involved in synthesizing Lewis a structure through the addition of fucose units onto the precursor glycan substrates.

Alterations in the Lewis antigen expression including Lewis a associate with gastric cancer and certain types of inflammatory disorders. Abnormal levels of this antigen can affect the binding of Helicobacter pylori contributing to gastric tissue changes and cancer development. Additionally changes in Lewis a expression can influence the interaction with proteins involved in cell adhesion during inflammatory responses potentially affecting conditions like inflammatory bowel disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Blood Group Lewis a

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 11: PubMed37631871

2023

Maternal and Infant Histo-Blood Group Antigen (HBGA) Profiles and Their Influence on Oral Rotavirus Vaccine (Rotarix) Immunogenicity among Infants in Zambia.

Applications

Unspecified application

Species

Unspecified reactive species

Adriace Chauwa,Samuel Bosomprah,Natasha Makabilo Laban,Bernard Phiri,Mwelwa Chibuye,Obvious Nchimunya Chilyabanyama,Sody Munsaka,Michelo Simuyandi,Innocent Mwape,Cynthia Mubanga,Masuzyo Chirwa Chobe,Caroline Chisenga,Roma Chilengi

Methods in molecular biology (Clifton, N.J.) 2460:33-44 PubMed34972929

2022

Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Sharad Purohit,Jin-Xiong She

Nature communications 9:258 PubMed29343722

2018

Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Sharad Purohit,Tiehai Li,Wanyi Guan,Xuezheng Song,Jing Song,Yanna Tian,Lei Li,Ashok Sharma,Boying Dun,David Mysona,Sharad Ghamande,Bunja Rungruang,Richard D Cummings,Peng George Wang,Jin-Xiong She

Cellular and molecular gastroenterology and hepatology 2:201-221.e15 PubMed26998508

2016

Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyuan Tang,Katie Partyka,Peter Hsueh,Jessica Y Sinha,Doron Kletter,Herbert Zeh,Ying Huang,Randall E Brand,Brian B Haab

Molecular & cellular proteomics : MCP 13:2944-60 PubMed25048705

2014

Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yu,Yi Lasanajak,Xuezheng Song,Liya Hu,Sasirekha Ramani,Megan L Mickum,David J Ashline,B V Venkataram Prasad,Mary K Estes,Vernon N Reinhold,Richard D Cummings,David F Smith

Biochimie 105:91-8 PubMed24998328

2014

Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells.

Applications

Unspecified application

Species

Human

Huiyu Zhuang,Zhenhua Hu,Mingzi Tan,Liancheng Zhu,Juanjuan Liu,Dawo Liu,Limei Yan,Bei Lin

Glycobiology 17:355-66 PubMed17202152

2007

Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Nyström,Ammi Grahn,Magnus Lindh,Maria Brytting,Ulla Mandel,Göran Larson,Sigvard Olofsson

Investigative ophthalmology & visual science 40:1631-6 PubMed10393028

1999

Alteration of sialyl Lewis epitope expression in pterygium.

Applications

Unspecified application

Species

Unspecified reactive species

C Creuzot-Garcher,V Guerzider,M Assem,A M Bron,P Delannoy,J Bara

Gastroenterology 102:424-30 PubMed1732113

1992

Lewis antigen alterations in gastric cancer precursors.

Applications

Unspecified application

Species

Unspecified reactive species

J Torrado,P Correa,B Ruiz,P Bernardi,D Zavala,J Bara

The Biochemical journal 254:185-93 PubMed2460087

1988

Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens.

Applications

Unspecified application

Species

Unspecified reactive species

J Bara,R Gautier,J Le Pendu,R Oriol

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Blood Group Lewis a antibody [7LE]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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