AB3968

Anti-Blood Group Lewis b antibody [2-25LE]

Name: Anti-Blood Group Lewis b antibody [2-25LE] (ab3968) | Abcam
Brand: Abcam Limited
SKU: AB3968
Price: 535 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(18 Publications)

Mouse Monoclonal Mucin 5AC antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MUC5AC.

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (AB3968)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (AB3968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling Blood Group Lewis b with ab3968 at 10 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2-25LE

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt, WB, ELISA, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human MUC5AC.

P98088

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Blood Group Lewis b also known as Le(b) is a carbohydrate antigen involved in the Lewis blood group system. Le(b) is formed by the addition of fucose to subterminal N-acetylglucosamine and galactose residues on type 1 precursor chains resulting in its mechanical function as a cell surface glycolipid. This antigen is about 650 Da in mass. Le(b) expresses predominantly on epithelial cells and secreted into body fluids by secretory glands including the stomach and the lungs.

Biological function summary

The Blood Group Lewis b plays a significant role in cell recognition and adhesion processes. It participates in immune response modulation by interacting with specific immune cells. Le(b) is not part of a major protein complex but interacts with other antigens to form distinct blood group phenotypes. The presence of Le(b) on cell surfaces can influence cellular interactions and immune recognition.

Pathways

Blood Group Lewis b plays a role in the fucosylation pathways which is a part of the larger glycosylation pathway. This influences cell-cell communication and signal transduction. The fucosylation of the Lewis antigens such as Le(b) is mediated by enzymes like fucosyltransferases which are important for the synthesis of these antigens. The relationship between Le(b) and other proteins in these pathways highlights its importance in maintaining cellular functions.

The presence and expression of Blood Group Lewis b antigen can impact conditions such as Helicobacter pylori infection and gastric cancer. The Le(b) antigen serves as a receptor for H. pylori facilitating bacterial adhesion to the gastric epithelium which may increase susceptibility to infection and subsequent disease progression. Proteins like fucosyltransferase involved in Le(b) biosynthesis may also be connected to altered expression patterns observed in gastric-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).

See full target information MUC5AC

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Immunobiology 227:152273 PubMed36088866

2022

Prevalence of high affinity naturally occurring IgG2 antibodies against human chorionic gonadotropin and its subunits in patients with ovarian cyst.

Applications

Unspecified application

Species

Unspecified reactive species

N Chikadze,M Tevzadze,M Janelidze,P Lydyard,N Porakishvili

Methods in molecular biology (Clifton, N.J.) 2460:33-44 PubMed34972929

2022

Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Sharad Purohit,Jin-Xiong She

BMC research notes 12:607 PubMed31547886

2019

Characterization of human norovirus binding to gut-associated bacterial ligands.

Applications

Unspecified application

Species

Unspecified reactive species

Erin A Almand,Matthew D Moore,Lee-Ann Jaykus

Oncotarget 9:34176-34188 PubMed30344930

2018

Aberrant O-glycosylation modulates aggressiveness in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hector A Cuello,Valeria I Segatori,Marina Albertó,Cynthia A Gulino,Rosario Aschero,Sandra Camarero,Laura Galluzzo Mutti,Kevin Madauss,Daniel F Alonso,Fabiana Lubieniecki,Mariano R Gabri

Nature communications 9:258 PubMed29343722

2018

Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Sharad Purohit,Tiehai Li,Wanyi Guan,Xuezheng Song,Jing Song,Yanna Tian,Lei Li,Ashok Sharma,Boying Dun,David Mysona,Sharad Ghamande,Bunja Rungruang,Richard D Cummings,Peng George Wang,Jin-Xiong She

Molecular & cellular proteomics : MCP 13:2944-60 PubMed25048705

2014

Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yu,Yi Lasanajak,Xuezheng Song,Liya Hu,Sasirekha Ramani,Megan L Mickum,David J Ashline,B V Venkataram Prasad,Mary K Estes,Vernon N Reinhold,Richard D Cummings,David F Smith

Biochimie 105:91-8 PubMed24998328

2014

Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells.

Applications

Unspecified application

Species

Human

Huiyu Zhuang,Zhenhua Hu,Mingzi Tan,Liancheng Zhu,Juanjuan Liu,Dawo Liu,Limei Yan,Bei Lin

PloS one 8:e54892 PubMed23408949

2013

Therapeutic targeting of Lewis(y) and Lewis(b) with a novel monoclonal antibody 692/29.

Applications

IHC-P, Flow Cyt

Species

Human, Human

Philip Noble,Ian Spendlove,Stephen Harding,Tina Parsons,Lindy G Durrant

Glycobiology 23:310-21 PubMed23144050

2012

Virus-induced appearance of the selectin ligand sLeX in herpes simplex virus type 1-infected T-cells: involvement of host and viral factors.

Applications

Flow Cyt

Species

Human

Rickard Nordén,Kristina Nyström,Johan Aurelius,Mikael Brisslert,Sigvard Olofsson

The Journal of biological chemistry 287:44784-99 PubMed23115247

2012

Functional glycomic analysis of human milk glycans reveals the presence of virus receptors and embryonic stem cell biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yu,Shreya Mishra,Xuezheng Song,Yi Lasanajak,Konrad C Bradley,Mary M Tappert,Gillian M Air,David A Steinhauer,Sujata Halder,Susan Cotmore,Peter Tattersall,Mavis Agbandje-McKenna,Richard D Cummings,David F Smith

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Blood Group Lewis b antibody [2-25LE]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (AB3968)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise