Mouse Monoclonal Blood Group Lewis y antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Blood Group Lewis y protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [LWY/1463] (AB219336), expandable thumbnail](https://content.abcam.com/products/images/blood-group-lewis-y-antibody-lwy-1463-ab219336--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38857.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [LWY/1463] (AB219336)")
Publications
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- International journal of molecular sciences 24:2023Features and Comparative Characteristics of Fucosylated Glycans Expression in Endothelial Glycocalyx of Placental Terminal Villi in Patients with Preeclampsia Treated with Different Antihypertensive Regimens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina M Ziganshina,Galina V Kulikova,Kamilla T Muminova,Alexander I Shchegolev,Ekaterina L Yarotskaya,Zulfiya S Khodzhaeva,Gennady T SukhikhPubMed 37958597
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing Blood Group Lewis y protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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1 product for Alternative Product
Alternative names
Lewis Y blood group
Recommended products
Mouse Monoclonal Blood Group Lewis y antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Blood Group Lewis y protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing Blood Group Lewis y protein. The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- LWY/1463
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Blood Group Lewis y also known as Le^y or the Lewis y antigen is a carbohydrate antigen found on the surface of red blood cells and certain epithelial tissues. It is a fucosylated oligosaccharide structure specifically an f3-Lewis structure with a molecular mass around 725 Daltons. The Lewis y antigen is expressed most strongly in the epithelial cells of the gastrointestinal tract lungs and urinary tract. It also appears in the endothelial cells of blood vessels and some tumor cells where it can act as a tumor-associated carbohydrate antigen.
- Biological function summary
The Lewis y antigen plays a significant role in cell adhesion and cell-cell recognition. It becomes engaged in the regulation of cellular interaction being part of complex glycoproteins and glycolipids on cell membranes. These structures serve as ligands for selectins which are adhesion molecules involved in immune responses. The presence of Lewis y antigen can influence cellular behavior and interactions impacting immune response and tumor progression.
- Pathways
The Lewis y antigen is involved in the selectin-mediated signaling pathway and glycosylation processes. These pathways relate to its role in cell adhesion and immune system navigation where it interacts with selectin proteins such as L-selectin and E-selectin. Glycosyltransferases enzymes part of the glycosylation process modify the Lewis y antigen forming structures essential for proper cellular recognition and communication in various physiological contexts.
- Associated diseases and disorders
The Lewis y antigen has associations with certain types of cancer such as epithelial ovarian and gastric cancers. It often becomes overexpressed in tumor cells making it a marker for cancer progression and a target for cancer immunotherapy. The upregulation of the Lewis y antigen might interact with molecules like HER2/neu a protein commonly overexpressed in cancer cells contributing to aggressive tumor behavior and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [LWY/1463] (ab219336), expandable thumbnail](https://content.abcam.com/products/images/blood-group-lewis-y-antibody-lwy-1463-ab219336--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38857.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [LWY/1463] (ab219336)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [LWY/1463] (ab219336)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung carcinoma tissue labeling Blood Group Lewis y with ab219336 at 1 μg/mL.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.
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