JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB219336

Anti-Blood Group Lewis y antibody [LWY/1463]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal Blood Group Lewis y antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Blood Group Lewis y protein.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [LWY/1463] (AB219336)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [LWY/1463] (AB219336)

Immunohistochemical analysis of formalin fixed paraffin embedded Human lung carcinoma tissue labeling Blood Group Lewis y with ab219336 at 1 μg/mL.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LWY/1463

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB3359

Anti-Blood Group Lewis y antibody [F3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB3649

Anti-Mucin 5AC antibody [45M1]

BOND RX™ Validated|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (AB3649)

  • 5
  • 8
Primary Antibodies

AB3359

Anti-Blood Group Lewis y antibody [F3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (AB3359)

  • 5
  • 1
Primary Antibodies

AB3967

Anti-Blood Group Lewis a antibody [7LE]

  • 5
  • 3
Primary Antibodies

AB223846

Anti-Gastric Mucin/MUC-6 antibody [EPR20623]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (AB223846)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Blood Group Lewis y also known as Le^y or the Lewis y antigen is a carbohydrate antigen found on the surface of red blood cells and certain epithelial tissues. It is a fucosylated oligosaccharide structure specifically an f3-Lewis structure with a molecular mass around 725 Daltons. The Lewis y antigen is expressed most strongly in the epithelial cells of the gastrointestinal tract lungs and urinary tract. It also appears in the endothelial cells of blood vessels and some tumor cells where it can act as a tumor-associated carbohydrate antigen.
Biological function summary

The Lewis y antigen plays a significant role in cell adhesion and cell-cell recognition. It becomes engaged in the regulation of cellular interaction being part of complex glycoproteins and glycolipids on cell membranes. These structures serve as ligands for selectins which are adhesion molecules involved in immune responses. The presence of Lewis y antigen can influence cellular behavior and interactions impacting immune response and tumor progression.

Pathways

The Lewis y antigen is involved in the selectin-mediated signaling pathway and glycosylation processes. These pathways relate to its role in cell adhesion and immune system navigation where it interacts with selectin proteins such as L-selectin and E-selectin. Glycosyltransferases enzymes part of the glycosylation process modify the Lewis y antigen forming structures essential for proper cellular recognition and communication in various physiological contexts.

The Lewis y antigen has associations with certain types of cancer such as epithelial ovarian and gastric cancers. It often becomes overexpressed in tumor cells making it a marker for cancer progression and a target for cancer immunotherapy. The upregulation of the Lewis y antigen might interact with molecules like HER2/neu a protein commonly overexpressed in cancer cells contributing to aggressive tumor behavior and metastasis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Blood Group Lewis y
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:136 PubMed40082910

2025

Basal cell adhesion molecule (BCAM) promotes mesothelial-to-mesenchymal transition and tumor angiogenesis through paracrine signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh Sivakumar,Sonja Lieber,Raimund Dietze,Vanessa M Beutgen,Eileen C Sutor,Sophie Heidemann,Florian Finkernagel,Julia Teply-Szymanski,Andrea Nist,Thorsten Stiewe,Katrin Roth,Silke Reinartz,Johannes Graumann,Sabine Müller-Brüsselbach,Rolf Müller

International journal of molecular sciences 24: PubMed37958597

2023

Features and Comparative Characteristics of Fucosylated Glycans Expression in Endothelial Glycocalyx of Placental Terminal Villi in Patients with Preeclampsia Treated with Different Antihypertensive Regimens.

Applications

Unspecified application

Species

Unspecified reactive species

Marina M Ziganshina,Galina V Kulikova,Kamilla T Muminova,Alexander I Shchegolev,Ekaterina L Yarotskaya,Zulfiya S Khodzhaeva,Gennady T Sukhikh
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com