Anti-Blooms Syndrome Protein Blm antibody (ab2179) is a rabbit polyclonal antibody that is used to detect Blooms Syndrome Protein Blm in Western Blot, IP. Suitable for Human, Mouse samples.
- Over 40 publications
- Trusted since 2002
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- Cell reports 42:1124282023Fanconi anemia-associated chromosomal radial formation is dependent on POLθ-mediated alternative end joining.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColette B Rogers,Rachel E Kram,Kevin Lin,Chad L Myers,Alexandra Sobeck,Eric A Hendrickson,Anja-Katrin BielinskyPubMed 37086407
- Communications biology 6:2342023PARP1 allows proper telomere replication through TRF1 poly (ADP-ribosyl)ation and helicase recruitment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Maresca,A Dello Stritto,C D'Angelo,E Petti,A Rizzo,E Vertecchi,F Berardinelli,L Bonanni,A Sgura,A Antoccia,G Graziani,A Biroccio,E SalvatiPubMed 36864251
- Cell reports 42:1120322023RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Kong,Zeyuan Liu,Ying Wai ChanPubMed 36719798
- Journal of cell communication and signaling 17:757-7722022BLM promotes malignancy in PCa by inducing KRAS expression and RhoA suppression via its interaction with HDGF and activation of MAPK/ERK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingchu Guo,Houqiang Xu,Mengqiu Huang,Yong RuanPubMed 36574142
- Nature communications 13:20122022A POLD3/BLM dependent pathway handles DSBs in transcribed chromatin upon excessive RNA:DNA hybrid accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Cohen,A Guenolé,I Lazar,A Marnef,T Clouaire,D V Vernekar,N Puget,V Rocher,C Arnould,M Aguirrebengoa,M Genais,N Firmin,R A Shamanna,R Mourad,V A Bohr,V Borde,G LegubePubMed 35440629
- Science advances 8:eabk02212022The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Balbo Pogliano,Ilaria Ceppi,Sara Giovannini,Vasiliki Petroulaki,Nathan Palmer,Federico Uliana,Marco Gatti,Kristina Kasaciunaite,Raimundo Freire,Ralf Seidel,Matthias Altmeyer,Petr Cejka,Joao MatosPubMed 35119917
- PLoS genetics 17:e10098682021The H3.3K27M oncohistone affects replication stress outcome and provokes genomic instability in pediatric glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrena Bočkaj,Tosca E I Martini,Eduardo S de Camargo Magalhães,Petra L Bakker,Tiny G J Meeuwsen-de Boer,Inna Armandari,Saskia L Meuleman,Marin T Mondria,Colin Stok,Yannick P Kok,Bjorn Bakker,René Wardenaar,Jonas Seiler,Mathilde J C Broekhuis,Hilda van den Bos,Diana C J Spierings,Femke C A Ringnalda,Hans Clevers,Ulrich Schüller,Marcel A T M van Vugt,Floris Foijer,Sophia W M BruggemanPubMed 34752469
- Nucleic acids research 49:11224-112402021USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenming Wu,Yiming Chang,Junliang Chen,Yang Su,Lei Li,Yuping Chen,Yunhui Li,Jinhuan Wu,Jinzhou Huang,Fei Zhao,Wenrui Wang,Hui Yin,Shunli Wang,Mingpeng Jin,Zhenkun Lou,Wei-Guo Zhu,Kuntian Luo,Jie Zhang,Jian YuanPubMed 34606619
- Cell death discovery 7:2602021ZGRF1 promotes end resection of DNA homologous recombination via forming complex with BRCA1/EXO1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Yan,Man Song,Jie Ping,Shu-Ting Lai,Xiao-Yu Cao,Chen-Jun Bai,Da-Fei Xie,Hua Guan,Shan-Shan Gao,Ping-Kun ZhouPubMed 34552057
- Molecular cell 81:2989-3006.e92021EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShashank Hambarde,Chi-Lin Tsai,Raj K Pandita,Albino Bacolla,Anirban Maitra,Vijay Charaka,Clayton R Hunt,Rakesh Kumar,Oliver Limbo,Remy Le Meur,Walter J Chazin,Susan E Tsutakawa,Paul Russell,Katharina Schlacher,Tej K Pandita,John A TainerPubMed 34197737
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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Target data
Function
ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction (PubMed:24816114, PubMed:25901030, PubMed:9388193, PubMed:9765292). Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288, PubMed:34606619). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed:25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA (PubMed:20639533, PubMed:24257077, PubMed:25901030). Unwinds G-quadruplex DNA; unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides (PubMed:18426915, PubMed:9765292). Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates (PubMed:9765292). Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich; formation of G-quadruplex DNA would block DNA replication and transcription (PubMed:18426915, PubMed:9765292). Negatively regulates sister chromatid exchange (SCE) (PubMed:25901030). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks (By similarity). (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration.
Alternative names
RECQ2, RECQL3, BLM, RecQ-like DNA helicase BLM, Bloom syndrome protein, DNA 3'-5' helicase BLM, RecQ protein-like 3, RecQ2
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Anti-Blooms Syndrome Protein Blm antibody (ab2179) is a rabbit polyclonal antibody that is used to detect Blooms Syndrome Protein Blm in Western Blot, IP. Suitable for Human, Mouse samples.
- Over 40 publications
- Trusted since 2002
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Blooms Syndrome Protein Blm antibody (ab2179) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), in Human, Mouse samples.
What is the molecular weight of Blooms Syndrome Protein Blm?
Anti-Blooms Syndrome Protein Blm (ab2179) specifically detects a band for Blooms Syndrome Protein Blm (UniProt: P54132) at a molecular weight of 159kDa.
Recommended positive controls
WB: HEK-293T, K-562, TCMK-1, NIH/3T3 and Jurkat whole cell lysate.IP: Blooms Syndrome Protein Blm in HEK-293T whole cell lysate.
Trusted by the scientific community
Anti-Blooms Syndrome Protein Blm (ab2179) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Blooms Syndrome Protein Blm (ab2179) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Blooms Syndrome Protein often abbreviated as BLM functions as a DNA helicase with a mass of approximately 141 kDa. BLM unwinds the DNA duplex important for genomic stability during replication and repair processes. This protein sometimes referred to as Bloom's Protein is expressed in various human tissues with higher levels in proliferating cells. BLM participates actively in the maintenance of genome integrity by resolving recombination intermediates and preventing crossover events.
- Biological function summary
The BLM helicase acts as a critical player in the maintenance of chromosomal stability. It forms part of a complex known as the BLM complex which includes topoisomerase IIIα RMI1 and RMI2. This complex participates in homologous recombination repair ensuring proper disjunction of intertwined DNA and preventing excessive sister chromatid exchanges. This function is essential for preventing genomic instability which may lead to cell dysfunction or malignancies.
- Pathways
The BLM protein associates with the DNA damage response and homologous recombination repair pathways. BLM interacts directly with components of the BRCA1-associated genome surveillance complex and indirectly with the ATM-dependent DNA damage signaling pathways to coordinate DNA repair processes. Its cooperation with proteins like RAD51 is integral for efficient DNA repair and recovery during replication stress conditions.
- Associated diseases and disorders
Bloom Syndrome directly relates to mutations or dysfunction in BLM. This rare genetic disorder leads to increased susceptibility to various cancers due to high rates of genomic instability and sister chromatid exchanges. In addition anomalies in BLM function may connect it to Werner syndrome where increased genomic instability is observed. Both conditions highlight BLM's role in safeguarding genome integrity and its critical interactions with proteins such as WRN helicase affecting cellular aging and carcinogenesis pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.
Antibodies: ab2179 used for IP at 6 μg per reaction.
Two lots tested (Lanes 1 and 2).
Lane 3 is Control IgG.
For blotting immunoprecipitated BLM, ab2179 was used at 0.1 μg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Predicted band size: 159 kDa
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/mL
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 15 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 5: A549 (Human lung carcinoma cell line) whole cell lysate at 15 µg
Predicted band size: 159 kDa
Exposure time: 30s
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/mL
Lane 1: TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 159 kDa
Exposure time: 75s
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