AB5446

Anti-Blooms Syndrome Protein Blm antibody

Name: Anti-Blooms Syndrome Protein Blm antibody (ab5446) | Abcam
Brand: Abcam Limited
SKU: AB5446
Rating: 5 (3 reviews)

(3 Reviews)

(2 Publications )

Goat Polyclonal Blooms Syndrome Protein Blm antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BLM.

View Alternative Names

RECQ2, RECQL3, BLM, RecQ-like DNA helicase BLM, Bloom syndrome protein, DNA 3'-5' helicase BLM, RecQ protein-like 3, RecQ2

2 Images

Supplier Data

Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (AB5446)

Blooms Syndrome Protein Blm was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab5446 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab5446 at 1 μg/ml.

Lane 1 : ab5446 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

Predicted band size: 159 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Blooms Syndrome Protein Blm antibody (AB5446)

All lanes:

Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446) at 0.04 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 159 kDa

false

Exposure time: 30s

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human BLM. The exact immunogen used to generate this antibody is proprietary information.

P54132

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Blooms Syndrome Protein often abbreviated as BLM functions as a DNA helicase with a mass of approximately 141 kDa. BLM unwinds the DNA duplex important for genomic stability during replication and repair processes. This protein sometimes referred to as Bloom's Protein is expressed in various human tissues with higher levels in proliferating cells. BLM participates actively in the maintenance of genome integrity by resolving recombination intermediates and preventing crossover events.

Biological function summary

The BLM helicase acts as a critical player in the maintenance of chromosomal stability. It forms part of a complex known as the BLM complex which includes topoisomerase IIIα RMI1 and RMI2. This complex participates in homologous recombination repair ensuring proper disjunction of intertwined DNA and preventing excessive sister chromatid exchanges. This function is essential for preventing genomic instability which may lead to cell dysfunction or malignancies.

Pathways

The BLM protein associates with the DNA damage response and homologous recombination repair pathways. BLM interacts directly with components of the BRCA1-associated genome surveillance complex and indirectly with the ATM-dependent DNA damage signaling pathways to coordinate DNA repair processes. Its cooperation with proteins like RAD51 is integral for efficient DNA repair and recovery during replication stress conditions.

Bloom Syndrome directly relates to mutations or dysfunction in BLM. This rare genetic disorder leads to increased susceptibility to various cancers due to high rates of genomic instability and sister chromatid exchanges. In addition anomalies in BLM function may connect it to Werner syndrome where increased genomic instability is observed. Both conditions highlight BLM's role in safeguarding genome integrity and its critical interactions with proteins such as WRN helicase affecting cellular aging and carcinogenesis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction (PubMed : 24816114, PubMed : 25901030, PubMed : 9388193, PubMed : 9765292). Participates in DNA replication and repair (PubMed : 12019152, PubMed : 21325134, PubMed : 23509288, PubMed : 34606619). Involved in 5'-end resection of DNA during double-strand break (DSB) repair : unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed : 21325134). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed : 25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA (PubMed : 20639533, PubMed : 24257077, PubMed : 25901030). Unwinds G-quadruplex DNA; unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides (PubMed : 18426915, PubMed : 9765292). Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates (PubMed : 9765292). Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich; formation of G-quadruplex DNA would block DNA replication and transcription (PubMed : 18426915, PubMed : 9765292). Negatively regulates sister chromatid exchange (SCE) (PubMed : 25901030). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks (By similarity).. (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration.

See full target information BLM

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:123 PubMed31913317

2020

Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation.

Applications

Unspecified application

Species

Unspecified reactive species

Clémence Mooser,Ioanna-Eleni Symeonidou,Pia-Amata Leimbacher,Alison Ribeiro,Ann-Marie K Shorrocks,Stephanie Jungmichel,Sara C Larsen,Katja Knechtle,Arti Jasrotia,Diana Zurbriggen,Alain Jeanrenaud,Colin Leikauf,Daniel Fink,Michael L Nielsen,Andrew N Blackford,Manuel Stucki

Molecular cell 74:571-583.e8 PubMed30898438

2019

MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Pia-Amata Leimbacher,Samuel E Jones,Ann-Marie K Shorrocks,Mara de Marco Zompit,Matthew Day,Jordy Blaauwendraad,Diana Bundschuh,Sarah Bonham,Roman Fischer,Daniel Fink,Benedikt M Kessler,Antony W Oliver,Laurence H Pearl,Andrew N Blackford,Manuel Stucki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Blooms Syndrome Protein Blm antibody

Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (AB5446)

Western blot - Anti-Blooms Syndrome Protein Blm antibody (AB5446)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise