AB62206

Anti-Blooms Syndrome Protein Blm (phospho T99) antibody

Name: Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206) | Abcam
Brand: Abcam Limited
SKU: AB62206
Price: 545 USD
Availability: InStock

(1 Review)

(2 Publications)

Rabbit Polyclonal Blooms Syndrome Protein Blm phospho T99 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BLM phospho T99 aa 50-150.

View Alternative Names

RECQ2, RECQL3, BLM, RecQ-like DNA helicase BLM, Bloom syndrome protein, DNA 3'-5' helicase BLM, RecQ protein-like 3, RecQ2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

ab62206 staining Blooms Syndrome Protein Blm (phospho T99) in Human heart by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The picture on the right is treated with the synthesized peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Immunofluorescence analysis of HeLa cells using Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206) at 1/500 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.

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Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

All lanes:

Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)

Lane 1:

HepG2 cell lysate

Lane 2:

HepG2 cell lysate with blocking peptide

Predicted band size: 159 kDa

Observed band size: 159 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human BLM phospho T99 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P54132

Specificity

ab62206 detects endogenous levels of Bloom Syndrome Protein Blm only when phosphorylated at threonine 99.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Blooms Syndrome Protein often abbreviated as BLM functions as a DNA helicase with a mass of approximately 141 kDa. BLM unwinds the DNA duplex important for genomic stability during replication and repair processes. This protein sometimes referred to as Bloom's Protein is expressed in various human tissues with higher levels in proliferating cells. BLM participates actively in the maintenance of genome integrity by resolving recombination intermediates and preventing crossover events.

Biological function summary

The BLM helicase acts as a critical player in the maintenance of chromosomal stability. It forms part of a complex known as the BLM complex which includes topoisomerase IIIα RMI1 and RMI2. This complex participates in homologous recombination repair ensuring proper disjunction of intertwined DNA and preventing excessive sister chromatid exchanges. This function is essential for preventing genomic instability which may lead to cell dysfunction or malignancies.

Pathways

The BLM protein associates with the DNA damage response and homologous recombination repair pathways. BLM interacts directly with components of the BRCA1-associated genome surveillance complex and indirectly with the ATM-dependent DNA damage signaling pathways to coordinate DNA repair processes. Its cooperation with proteins like RAD51 is integral for efficient DNA repair and recovery during replication stress conditions.

Bloom Syndrome directly relates to mutations or dysfunction in BLM. This rare genetic disorder leads to increased susceptibility to various cancers due to high rates of genomic instability and sister chromatid exchanges. In addition anomalies in BLM function may connect it to Werner syndrome where increased genomic instability is observed. Both conditions highlight BLM's role in safeguarding genome integrity and its critical interactions with proteins such as WRN helicase affecting cellular aging and carcinogenesis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction (PubMed : 24816114, PubMed : 25901030, PubMed : 9388193, PubMed : 9765292). Participates in DNA replication and repair (PubMed : 12019152, PubMed : 21325134, PubMed : 23509288, PubMed : 34606619). Involved in 5'-end resection of DNA during double-strand break (DSB) repair : unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed : 21325134). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed : 25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA (PubMed : 20639533, PubMed : 24257077, PubMed : 25901030). Unwinds G-quadruplex DNA; unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides (PubMed : 18426915, PubMed : 9765292). Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates (PubMed : 9765292). Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich; formation of G-quadruplex DNA would block DNA replication and transcription (PubMed : 18426915, PubMed : 9765292). Negatively regulates sister chromatid exchange (SCE) (PubMed : 25901030). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks (By similarity).. (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration.

See full target information BLM phospho T99

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 20:251 PubMed32565735

2020

Random-forest algorithm based biomarkers in predicting prognosis in the patients with hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Guo,Zhenjiang Wang,Yuanyuan Du,Jie Mao,Junqiang Zhang,Zeyuan Yu,Jiwu Guo,Jun Zhao,Huinian Zhou,Haitao Wang,Yanmei Gu,Yumin Li

Nature communications 9:1016 PubMed29523790

2018

MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Tripathi,Himanshi Agarwal,Swati Priya,Harish Batra,Priyanka Modi,Monica Pandey,Dhurjhoti Saha,Sathees C Raghavan,Sagar Sengupta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Blooms Syndrome Protein Blm (phospho T99) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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