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AB115251

Anti-Bmi1 antibody

5

(1 Review)

|

(4 Publications)

Goat Polyclonal BMI1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human BMI1 aa 250-300.

View Alternative Names

PCGF4, RNF51, BMI1, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody (AB115251)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody (AB115251)

Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with ab115251 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody (AB115251)
  • IHC-P

AbReview63346****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody (AB115251)

ab115251 staining Bmi1 in Mouse Pancreatic intraepithelial neoplasia tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% BSA for 60 minutes at 26°C and antigen retrieval was by heat mediation in Tris-based buffer. The sample was incubated with primary antibody (1/200 in 10% BSA in TBST) at 4°C for 16 hours. A HRP-conjugated Donkey anti-goatpolyclonal (1/1000) was used as the secondary antibody.

This image is courtesy of an abreview by Wenjian Cao

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human BMI1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P35226

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bmi1 also known as B-cell-specific Moloney murine leukemia virus integration site 1 is a member of the Polycomb group of proteins. It has an approximate molecular mass of 37 kDa. Bmi1 is expressed in many tissues including the brain bone marrow and hematopoietic cells. It functions as a transcriptional repressor. Bmi1 plays an important role in chromatin remodeling influencing gene silencing and regulation of developmental processes. Bmi1 ELISA assays are commonly used for quantifying its levels in various research applications.
Biological function summary

The functions of Bmi1 extend into maintaining stem cell renewal and differentiation. It acts as part of the Polycomb repressive complex 1 (PRC1) partnering with other proteins to regulate gene expression by altering chromatin structure. Bmi1 protein regulates cell proliferation and inhibits senescence by repressing the INK4a/ARF locus which encodes tumor suppressor proteins like p16INK4a and p14ARF.

Pathways

Bmi1 plays significant roles in processes such as the DNA damage response and the self-renewal of hematopoietic stem cells. It is involved in the modulation of the Wnt and Notch signaling pathways. Bmi1 protein interacts closely with proteins such as CBX2 and RNF2 within these pathways affecting the transcriptional landscape that governs cell fate decisions.

Bmi1 overexpression links to cancer particularly breast cancer and leukemia. In MCF7 breast cancer cells alterations in Bmi1 activity contribute to oncogenesis and tumor progression. Its dysregulation can connect to Myc a well-known oncogenic protein. Additionally Bmi1's influence in Cas9-based gene editing models highlights its potential in cancer therapy research. Bmi1 has also been implicated in neurodegenerative disorders with connections to proteins like p21CIP1 affecting neuronal survival and aging.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 15386022, PubMed : 16359901, PubMed : 16714294, PubMed : 21772249, PubMed : 25355358, PubMed : 26151332, PubMed : 27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed : 21772249, PubMed : 25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed : 15386022, PubMed : 21772249, PubMed : 26151332).
See full target information BMI1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37511456

2023

A Short-Term Model of Colitis-Associated Colorectal Cancer That Suggests Initial Tumor Development and the Characteristics of Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yasushi Matsumoto,Toshiro Fukui,Shunsuke Horitani,Yuji Tanimura,Ryo Suzuki,Takashi Tomiyama,Yusuke Honzawa,Tomomitsu Tahara,Kazuichi Okazaki,Makoto Naganuma

Oncology letters 21:7 PubMed33240413

2020

Long-term model of colitis-associated colorectal cancer suggests tumor spread mechanism and nature of cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Tanimura,Toshiro Fukui,Shunsuke Horitani,Yasushi Matsumoto,Sachi Miyamoto,Ryo Suzuki,Toshihiro Tanaka,Takashi Tomiyama,Tsukasa Ikeura,Yugo Ando,Akiyoshi Nishio,Kazuichi Okazaki

The Journal of pathology 248:179-190 PubMed30689202

2019

Bmi1 marks gastric stem cells located in the isthmus in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Takuto Yoshioka,Akihisa Fukuda,Osamu Araki,Satoshi Ogawa,Yuta Hanyu,Yoshihide Matsumoto,Yuichi Yamaga,Yuki Nakanishi,Kenji Kawada,Yoshiharu Sakai,Tsutomu Chiba,Hiroshi Seno

Experimental and therapeutic medicine 13:1329-1336 PubMed28413473

2017

MicroRNA-200c suppresses cell growth and metastasis by targeting Bmi-1 and E2F3 in renal cancer cells.

Applications

WB

Species

Human

Mingning Qiu,Ziji Liang,Lieqian Chen,Guobin Tan,Lei Liu,Kangning Wang,Hege Chen,Jianjun Liu
View all publications
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Product promise

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