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AB38295

Anti-Bmi1 antibody

4

(5 Reviews)

|

(46 Publications)

Rabbit Polyclonal BMI1 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 46 publications. Immunogen corresponding to Synthetic Peptide within Human BMI1.

View Alternative Names

PCGF4, RNF51, BMI1, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody (AB38295)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody (AB38295)

Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.

Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody (AB38295)
  • ICC/IF

AbReview39022****

Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody (AB38295)

ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Western blot - Anti-Bmi1 antibody (AB38295)
  • WB

Unknown

Western blot - Anti-Bmi1 antibody (AB38295)

Lane 1:

Western blot - Anti-Bmi1 antibody (ab38295) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Bmi1 antibody (ab38295) at 1 µg/mL

Lane 3:

Western blot - Anti-Bmi1 antibody (ab38295) at 2 µg/mL

All lanes:

K562 cell lysate

Predicted band size: 36 kDa

Observed band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

IP, IHC-Fr, ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human BMI1. The exact immunogen used to generate this antibody is proprietary information.

P35226

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bmi1 also known as B-cell-specific Moloney murine leukemia virus integration site 1 is a member of the Polycomb group of proteins. It has an approximate molecular mass of 37 kDa. Bmi1 is expressed in many tissues including the brain bone marrow and hematopoietic cells. It functions as a transcriptional repressor. Bmi1 plays an important role in chromatin remodeling influencing gene silencing and regulation of developmental processes. Bmi1 ELISA assays are commonly used for quantifying its levels in various research applications.
Biological function summary

The functions of Bmi1 extend into maintaining stem cell renewal and differentiation. It acts as part of the Polycomb repressive complex 1 (PRC1) partnering with other proteins to regulate gene expression by altering chromatin structure. Bmi1 protein regulates cell proliferation and inhibits senescence by repressing the INK4a/ARF locus which encodes tumor suppressor proteins like p16INK4a and p14ARF.

Pathways

Bmi1 plays significant roles in processes such as the DNA damage response and the self-renewal of hematopoietic stem cells. It is involved in the modulation of the Wnt and Notch signaling pathways. Bmi1 protein interacts closely with proteins such as CBX2 and RNF2 within these pathways affecting the transcriptional landscape that governs cell fate decisions.

Bmi1 overexpression links to cancer particularly breast cancer and leukemia. In MCF7 breast cancer cells alterations in Bmi1 activity contribute to oncogenesis and tumor progression. Its dysregulation can connect to Myc a well-known oncogenic protein. Additionally Bmi1's influence in Cas9-based gene editing models highlights its potential in cancer therapy research. Bmi1 has also been implicated in neurodegenerative disorders with connections to proteins like p21CIP1 affecting neuronal survival and aging.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 15386022, PubMed : 16359901, PubMed : 16714294, PubMed : 21772249, PubMed : 25355358, PubMed : 26151332, PubMed : 27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed : 21772249, PubMed : 25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed : 15386022, PubMed : 21772249, PubMed : 26151332).
See full target information BMI1
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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & therapeutics 33:842-851 PubMed40765264

2025

An Endolichenic Fungi-Derived Fatty Acid, cis-10-Nonadecenoic acid, Suppresses Colorectal Cancer Stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Mücahit Varlı,Eun-Young Lee,So-Yeon Park,Yi Yang,Prima F Hillman,Rui Zhou,Jae-Seoun Hur,Sang-Jip Nam,Hangun Kim

Scientific reports 14:11008 PubMed38744845

2024

CBX7 silencing promoted liver regeneration by interacting with BMI1 and activating the Nrf2/ARE signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Dou,Fei Lu,Jinjing Hu,Bin Li,Xun Li

Scientific reports 13:2811 PubMed36797277

2023

1'-O-methyl-averantin isolated from the endolichenic fungus Jackrogersella sp. EL001672 suppresses colorectal cancer stemness via sonic Hedgehog and Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mücahit Varlı,Eun-Young Lee,Yi Yang,Rui Zhou,İsa Taş,Sultan Pulat,Chathurika D B Gamage,So-Yeon Park,Jae-Seoun Hur,Sang-Jip Nam,Hangun Kim

Journal of animal science and technology 64:1105-1116 PubMed36812001

2022

Establishment of intestinal organoids from small intestine of growing cattle (12 months old).

Applications

Unspecified application

Species

Unspecified reactive species

Kang Won Park,Hyeon Yang,Min Gook Lee,Sun A Ock,Hayeon Wi,Poongyeon Lee,In-Sul Hwang,Jae Gyu Yoo,Choon-Keun Park,Bo Ram Lee

Cancers 14: PubMed36077751

2022

Stage-Specific Effect of Inositol Hexaphosphate on Cancer Stem Cell Pool during Growth and Progression of Prostate Tumorigenesis in TRAMP Model.

Applications

Unspecified application

Species

Unspecified reactive species

Komal Raina,Kushal Kandhari,Anil K Jain,Kameswaran Ravichandran,Paul Maroni,Chapla Agarwal,Rajesh Agarwal

International journal of molecular sciences 22: PubMed34360579

2021

Bmi1 Augments Proliferation and Survival of Cortical Bone-Derived Stem Cells after Injury through Novel Epigenetic Signaling via Histone 3 Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay Kraus,Chris Bryan,Marcus Wagner,Tabito Kino,Melissa Gunchenko,Wassy Jalal,Mohsin Khan,Sadia Mohsin

Experimental and therapeutic medicine 21:619 PubMed33936276

2021

Polycomb group protein Bmi1 is required for the neuronal differentiation of mouse induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Shan,Liping Zhou,Lizhen Liu,Deju Lin,Qin Yu

Stem cell research & therapy 12:169 PubMed33691791

2021

Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaoying Jia,Yan Cui,Zhigang Tan,Min Liu,Yugang Jiang

Oncology letters 21:98 PubMed33376531

2020

Hypoxic exposure activates the B cell-specific Moloney murine leukaemia virus integration site 1/PI3K/Akt axis and promotes EMT in leukaemia stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyan Jiang,Guoqian He,Jianhua Li,Jinrong Li,Xia Guo,Ju Gao

Oncology reports 44:2691-2700 PubMed33125503

2020

MicroRNA‑487b‑3p inhibits osteosarcoma chemoresistance and metastasis by targeting ALDH1A3.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Cheng,Ping-Guo Duan,Zhi-Zeng Gao,Min Dai
View all publications
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