Goat Polyclonal BMP1/PCP antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human BMP1 aa 250-300.
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- The European respiratory journal 61:2023Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuju Wu,Daoqin Zhang,Xiaojing Qiao,Li Zhang,Xinjiang Cai,Jaden Ji,Jocelyn A Ma,Yan Zhao,John A Belperio,Kristina I Boström,Yucheng YaoPubMed 36758986
- Nature communications 13:49432022scRNA-seq of gastric tumor shows complex intercellular interaction with an alternative T cell exhaustion trajectory.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeyong Sun,Runda Xu,Fuhai Ma,Naixue Yang,Yang Li,Xiaofeng Sun,Peng Jin,Wenzhe Kang,Lemei Jia,Jianping Xiong,Haitao Hu,Yantao Tian,Xun LanPubMed 35999201
- Aging 13:22528-225432021Inhibition of FSTL3 abates the proliferation and metastasis of renal cell carcinoma via the GSK-3β/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengfeng Sun,Peng Sun,Xiaofeng Yang,Liangliang Hu,Jianguo Gao,Tao TianPubMed 34555811
- Heliyon 6:e043472020Local SDF-1α application enhances the therapeutic efficacy of BMSCs transplantation in osteoporotic bone healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Liu et. al.PubMed 32637715
- Scientific reports 9:186352019Bone secreted factors induce cellular quiescence in prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Yuan Yu-Lee et. al.PubMed 31819067
- Cancer gene therapy 27:330-3402019Overexpression of BMP1 reflects poor prognosis in clear cell renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Xiao et. al.PubMed 31155610
- Biomedical reports 11:27-302019Immunohistochemical analyses to determine pathogenesis of tenosynovitis with psammomatous calcification in the wrist: A case report.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChika Miyasaka et. al.PubMed 31281641
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BMP1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P13497
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 6 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Metalloprotease that plays key roles in regulating the formation of the extracellular matrix (ECM) via processing of various precursor proteins into mature functional enzymes or structural proteins (PubMed:33206546). Thereby participates in several developmental and physiological processes such as cartilage and bone formation, muscle growth and homeostasis, wound healing and tissue repair (PubMed:32636307, PubMed:33169406). Roles in ECM formation include cleavage of the C-terminal propeptides from procollagens such as procollagen I, II and III or the proteolytic activation of the enzyme lysyl oxidase LOX, necessary to formation of covalent cross-links in collagen and elastic fibers (PubMed:31152061, PubMed:33206546). Additional substrates include matricellular thrombospondin-1/THBS1 whose cleavage leads to cell adhesion disruption and TGF-beta activation (PubMed:32636307). Isoform BMP1-3. Plays an important role in bone repair by acting as a coactivator of BMP7.
Alternative names
PCOLC, BMP1, Bone morphogenetic protein 1, BMP-1, Mammalian tolloid protein, Procollagen C-proteinase, mTld, PCP
Recommended products
Goat Polyclonal BMP1/PCP antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human BMP1 aa 250-300.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BMP1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P13497
- Purification technique
- Affinity purification Immunogen
- Specificity
ab205394 is expected to recognize both reported isoforms (NP_001190.1; NP_006120.1).
- Concentration
- Purification notes
ab205394 was purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP1/PCP antibody (ab205394)
Immunohistochemical analysis of paraffin embedded Human placenta tissue, staining BMP1/PCP with ab205394 at 6µg/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP1/PCP antibody (ab205394)
Immunohistochemical analysis of paraffin embedded Human colon tissue, staining BMP1/PCP with ab205394 at 6µg/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Western blot - Anti-BMP1/PCP antibody (ab205394)
Detected by chemiluminescence.
Lanes 1, 3, 4, 5 and 6: Western blot - Anti-BMP1/PCP antibody (ab205394) at 2 µg/mL
Lane 2: Western blot - Anti-BMP1/PCP antibody (ab205394) at 1 µg/mL
Lane 1: Jurkat cell lysate at 35 µg
Lane 2: A549 cell lysate at 35 µg
Lane 3: MCF7 cell lysate at 35 µg
Lane 4: HeLa cell lysate at 35 µg
Lane 5: NIH3T3 cell lysate at 35 µg
Lane 6: PC12 cell lysate at 35 µg
Predicted band size: 111 kDa
Western blot - Anti-BMP1/PCP antibody (ab205394)
Detected by chemiluminescence.
All lanes: Western blot - Anti-BMP1/PCP antibody (ab205394) at 1 µg/mL
All lanes: Human Liver lysate at 35 µg
Predicted band size: 111 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-BMP1/PCP antibody (ab205394)
BMP1/PCP western blot using anti-BMP1/PCP antibody ab205394. Publication image and figure legend from Xiao, W., Wang, X., et al., 2020, Cancer Gene Ther, PubMed 31155610.
ab205394 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab205394 please see the product overview.
Downregulation of BMP1 suppresses malignancy of ccRCC in vitro. a, b BMP1 is upregulated in ccRCC tissues and renal cancer cells by immunohistochemistry. c, d Silence BMP1 with shRNA (sh-BMP1-1 and sh-BMP1-2). e Silence BMP1 significantly repressed the cell proliferation of A498 and Caki-1 cells. f Silence BMP1 significantly repressed the migration and invasion of Caki-1 cells. Data indicate the means ± SEM. *P < 0.05. **P < 0.01
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