AB205394

Anti-BMP1/PCP antibody

Name: Anti-BMP1/PCP antibody (ab205394) | Abcam
Brand: Abcam Limited
SKU: AB205394
Price: 525 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(10 Publications)

Goat Polyclonal BMP1/PCP antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human BMP1 aa 250-300.

View Alternative Names

PCOLC, BMP1, Bone morphogenetic protein 1, BMP-1, Mammalian tolloid protein, Procollagen C-proteinase, mTld, PCP

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP1/PCP antibody (AB205394)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP1/PCP antibody (AB205394)

Immunohistochemical analysis of paraffin embedded Human colon tissue, staining BMP1/PCP with ab205394 at 6µg/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP1/PCP antibody (AB205394)

Immunohistochemical analysis of paraffin embedded Human placenta tissue, staining BMP1/PCP with ab205394 at 6µg/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Supplier Data

Western blot - Anti-BMP1/PCP antibody (AB205394)

Detected by chemiluminescence.

Lanes 1, 3, 4, 5 and 6:

Western blot - Anti-BMP1/PCP antibody (ab205394) at 2 µg/mL

Lane 2:

Western blot - Anti-BMP1/PCP antibody (ab205394) at 1 µg/mL

Lane 1:

Jurkat cell lysate at 35 µg

Lane 2:

A549 cell lysate at 35 µg

Lane 3:

MCF7 cell lysate at 35 µg

Lane 4:

HeLa cell lysate at 35 µg

Lane 5:

NIH3T3 cell lysate at 35 µg

Lane 6:

PC12 cell lysate at 35 µg

Predicted band size: 111 kDa

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Supplier Data

Western blot - Anti-BMP1/PCP antibody (AB205394)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-BMP1/PCP antibody (ab205394) at 1 µg/mL

All lanes:

Human Liver lysate at 35 µg

Predicted band size: 111 kDa

false

CiteAb

Western blot - Anti-BMP1/PCP antibody (AB205394)

BMP1/PCP western blot using anti-BMP1/PCP antibody ab205394. Publication image and figure legend from Xiao, W., Wang, X., et al., 2020, Cancer Gene Ther, PubMed 31155610.

ab205394 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab205394 please see the product overview.

Downregulation of BMP1 suppresses malignancy of ccRCC in vitro. a, b BMP1 is upregulated in ccRCC tissues and renal cancer cells by immunohistochemistry. c, d Silence BMP1 with shRNA (sh-BMP1-1 and sh-BMP1-2). e Silence BMP1 significantly repressed the cell proliferation of A498 and Caki-1 cells. f Silence BMP1 significantly repressed the migration and invasion of Caki-1 cells. Data indicate the means ± SEM. *p < 0.05. **p < 0.01

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human BMP1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P13497

Specificity

ab205394 is expected to recognize both reported isoforms (NP_001190.1; NP_006120.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab205394 was purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloprotease that plays key roles in regulating the formation of the extracellular matrix (ECM) via processing of various precursor proteins into mature functional enzymes or structural proteins (PubMed : 33206546). Thereby participates in several developmental and physiological processes such as cartilage and bone formation, muscle growth and homeostasis, wound healing and tissue repair (PubMed : 32636307, PubMed : 33169406). Roles in ECM formation include cleavage of the C-terminal propeptides from procollagens such as procollagen I, II and III or the proteolytic activation of the enzyme lysyl oxidase LOX, necessary to formation of covalent cross-links in collagen and elastic fibers (PubMed : 31152061, PubMed : 33206546). Additional substrates include matricellular thrombospondin-1/THBS1 whose cleavage leads to cell adhesion disruption and TGF-beta activation (PubMed : 32636307).. Isoform BMP1-3. Plays an important role in bone repair by acting as a coactivator of BMP7.

See full target information BMP1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 125:e30609 PubMed38860429

2024

BMP1 Promotes Keloid by Inducing Fibroblast Inflammation and Fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Yahui Chen,Jinfeng Wu,Xiangguang Shi

Journal of orthopaedic surgery and research 19:146 PubMed38369459

2024

Periostin/Bone Morphogenetic Protein 1 axis axis regulates proliferation and osteogenic differentiation of sutured mesenchymal stem cells and affects coronal suture closure in the TWIST1 mouse model of craniosynostosis.

Applications

Unspecified application

Species

Unspecified reactive species

ShuBin Feng,Qiang Feng,LiuJian Dong,Qiang Lv,ShiYue Mei,YaoDong Zhang

Scientific reports 13:10978 PubMed37414855

2023

Molecular determinants of epithelial mesenchymal transition in mouse placenta and trophoblast stem cell.

Applications

Unspecified application

Species

Unspecified reactive species

Shipra Kanti Jena,Shreya Das,Shreeta Chakraborty,Rupasri Ain

The European respiratory journal 61: PubMed36758986

2023

Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuju Wu,Daoqin Zhang,Xiaojing Qiao,Li Zhang,Xinjiang Cai,Jaden Ji,Jocelyn A Ma,Yan Zhao,John A Belperio,Kristina I Boström,Yucheng Yao

Nature communications 13:4943 PubMed35999201

2022

scRNA-seq of gastric tumor shows complex intercellular interaction with an alternative T cell exhaustion trajectory.

Applications

Unspecified application

Species

Unspecified reactive species

Keyong Sun,Runda Xu,Fuhai Ma,Naixue Yang,Yang Li,Xiaofeng Sun,Peng Jin,Wenzhe Kang,Lemei Jia,Jianping Xiong,Haitao Hu,Yantao Tian,Xun Lan

Aging 13:22528-22543 PubMed34555811

2021

Inhibition of FSTL3 abates the proliferation and metastasis of renal cell carcinoma via the GSK-3β/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fengfeng Sun,Peng Sun,Xiaofeng Yang,Liangliang Hu,Jianguo Gao,Tao Tian

Heliyon 6:e04347 PubMed32637715

2020

Local SDF-1α application enhances the therapeutic efficacy of BMSCs transplantation in osteoporotic bone healing.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Yi Wen,Jun Qiu,Zhaoyichun Zhang,Zuolin Jin,Meng Cao,Yang Jiao,Hongxu Yang

Scientific reports 9:18635 PubMed31819067

2019

Bone secreted factors induce cellular quiescence in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Yuan Yu-Lee,Yu-Chen Lee,Jing Pan,Song-Chang Lin,Tianhong Pan,Guoyu Yu,David H Hawke,Bih-Fang Pan,Sue-Hwa Lin

Cancer gene therapy 27:330-340 PubMed31155610

2019

Overexpression of BMP1 reflects poor prognosis in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Xiao,Xuegang Wang,Tao Wang,Jinchun Xing

Biomedical reports 11:27-30 PubMed31281641

2019

Immunohistochemical analyses to determine pathogenesis of tenosynovitis with psammomatous calcification in the wrist: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Chika Miyasaka,Mitsuaki Ishida,Yoshitaka Hamada,Takeyasu Toyama,Koji Tsuta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BMP1/PCP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP1/PCP antibody (AB205394)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP1/PCP antibody (AB205394)

Western blot - Anti-BMP1/PCP antibody (AB205394)

Western blot - Anti-BMP1/PCP antibody (AB205394)

Western blot - Anti-BMP1/PCP antibody (AB205394)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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