Rabbit Recombinant Monoclonal BMP2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (AB225898), expandable thumbnail](https://content.abcam.com/products/images/bmp2-antibody-epr20807-bsa-and-azide-free-ab225898--immunocytochemistry-immunofluorescence-img111057.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (AB225898)")
![Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (AB225898), expandable thumbnail](https://content.abcam.com/products/images/bmp2-antibody-epr20807-bsa-and-azide-free-ab225898--flow-cytometry-intracellular-img6322.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (AB225898)")
![Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (AB225898), expandable thumbnail](https://content.abcam.com/products/images/bmp2-antibody-epr20807-bsa-and-azide-free-ab225898--immunocytochemistry-immunofluorescence-img19271.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (AB225898)")
Publications
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- Frontiers in cell and developmental biology 9:6426462021miR-27a-5p-Abundant Small Extracellular Vesicles Derived From Epimedium-Preconditioned Bone Mesenchymal Stem Cells Stimulate Osteogenesis by Targeting Atg4B-Mediated Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyun Li,Rumeng Chen,Yunchuan Li,Panpan Wang,Yan Cui,Li Yang,Xiaofeng Zhu,Ronghua ZhangPubMed 34621733
- Frontiers in molecular biosciences 8:6793452021miR-330-5p in Small Extracellular Vesicles Derived From Plastrum testudinis-Preconditioned Bone Mesenchymal Stem Cells Attenuates Osteogenesis by Modulating Wnt/β-Catenin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyun Li,Yan Cui,Qing Lin,Panpan Wang,Rumeng Chen,Xiaofeng Zhu,Li Yang,Ronghua ZhangPubMed 34434963
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Expected | Predicted | Predicted |
| Mouse | Expected | Tested | Tested |
| Rat | Expected | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:31019025, PubMed:24362451). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:20851880). Stimulates also the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451).
Alternative names
Bone morphogenetic protein 2, BMP-2, Bone morphogenetic protein 2A, BMP-2A, BMP2A, BMP2
Recommended products
Rabbit Recombinant Monoclonal BMP2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20807
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab225898 is the carrier-free version of Anti-BMP2 antibody [EPR20807] ab214821.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898), expandable thumbnail](https://content.abcam.com/products/images/bmp2-antibody-epr20807-bsa-and-azide-free-ab225898--immunocytochemistry-immunofluorescence-img111057.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)")
Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling BMP2 with Anti-BMP2 antibody [EPR20807] ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LNCaP cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BMP2 antibody [EPR20807] ab214821).
![Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898), expandable thumbnail](https://content.abcam.com/products/images/bmp2-antibody-epr20807-bsa-and-azide-free-ab225898--flow-cytometry-intracellular-img6322.jpg "Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)")
Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling BMP2 with Anti-BMP2 antibody [EPR20807] ab214821 at 1/700 dilution (red) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BMP2 antibody [EPR20807] ab214821).
![Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898), expandable thumbnail](https://content.abcam.com/products/images/bmp2-antibody-epr20807-bsa-and-azide-free-ab225898--immunocytochemistry-immunofluorescence-img19271.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)")
Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] - BSA and Azide free (ab225898)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling BMP2 with Anti-BMP2 antibody [EPR20807] ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BMP2 antibody [EPR20807] ab214821).
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