Anti-BMP4 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (AB235114)

Paraffin-embedded human lung tissue stained for BMP4 using ab235114 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (AB235114)

Paraffin-embedded human prostate cancer tissue stained for BMP4 using ab235114 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (AB235114)

Paraffin-embedded human small intestine tissue stained for BMP4 using ab235114 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (AB235114)

Paraffin-embedded human appendix tissue stained for BMP4 using ab235114 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

• WB

Supplier Data

Western blot - Anti-BMP4 antibody (AB235114)

All lanes:

Western blot - Anti-BMP4 antibody (ab235114) at 1/500 dilution

Lane 1:

Mouse kidney lysate

Lane 2:

Mouse liver lysate

Lane 3:

Rat liver lysate

Lane 4:

Rat kidney lysate

Lane 5:

Whole Zebrafish lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-BMP4 antibody (AB235114)

All lanes:

Western blot - Anti-BMP4 antibody (ab235114) at 1/500 dilution

Lane 1:

Rat liver lysate

Lane 2:

A549 (human lung carcinoma cell line) cell lysate

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-BMP4 antibody (AB235114)

All lanes:

Western blot - Anti-BMP4 antibody (ab235114) at 1/500 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Mouse skeletal muscle lysate

Lane 3:

Whole Zebrafish lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 47 kDa

false