Anti-BMP4 antibody

• WB

Ap

Western blot - Anti-BMP4 antibody (AB39973)

ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-BMP4 antibody (ab39973) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Mouse Tissue Lysate at 10 µg

Lane 3:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 4:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 5:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 6:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg with Human BMP4 peptide (<a href='/en-us/products/unavailable/human-bmp4-peptide-ab40140'>ab40140</a>)

Lane 8:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg with Human BMP4 peptide (<a href='/en-us/products/unavailable/human-bmp4-peptide-ab40140'>ab40140</a>)

Lane 9:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg with Human BMP4 peptide (<a href='/en-us/products/unavailable/human-bmp4-peptide-ab40140'>ab40140</a>)

Lane 10:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg with Human BMP4 peptide (<a href='/en-us/products/unavailable/human-bmp4-peptide-ab40140'>ab40140</a>)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 100 kDa,47 kDa

false

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (AB39973)

ICC/IF image of ab39973 staining primary Rat hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

• WB

Unknown

Western blot - Anti-BMP4 antibody (AB39973)

All lanes:

Western blot - Anti-BMP4 antibody (ab39973) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 47 kDa

Observed band size: 42 kDa,47 kDa

false

• WB

Unknown

Western blot - Anti-BMP4 antibody (AB39973)

All lanes:

Western blot - Anti-BMP4 antibody (ab39973) at 1/1000 dilution

All lanes:

mouse brain at 20 µg

Secondary

All lanes:

Dako Cytomation P0260 Conjugation: HRP at 1/3000 dilution

Predicted band size: 47 kDa

true

Exposure time: 1min

This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom