Rabbit Polyclonal BMP4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 98 publications.
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- Disease models & mechanisms 16:2023Disrupting Hedgehog signaling in melanocytes by SUFU knockout leads to ocular melanocytosis and anterior segment malformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeizhuo Wang,Feiyang Li,Jing Wang,Zuimeng Liu,Meiyu Tian,Zhenhang Wang,Huirong Li,Jia Qu,Yu Chen,Ling HouPubMed 37577930
- Joint diseases and related surgery 34:413-4242023The effects of cephalexin on fracture healing in a rat femur fracture model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammed Uslu,Bilal Yılmaz,Hamisi Mwarindano Mraja,Mustafa Fatih Daşcı,Elif Yaprak Saraç,Bedri Onur Küçükyıldırım,Mehmet Akif Güleç,Serdar YükselPubMed 37462646
- iScience 26:1062722023-mediated angiogenesis facilitates the beiging of white adipose tissue in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenguang Wang,Yalan Wu,Yangxian Li,Yang Zhang,Yee Lok Fung,Ka Kui Tong,Chi Wai Lau,Li Xiang,Kin Ming Kwan,Li-Ru You,Yu Huang,Xiao Yu TianPubMed 36915676
- Cellular and molecular life sciences : CMLS 80:582023Hypermethylation of the Bmp4 promoter dampens binding of HIF-1α and impairs its cardiac protective effects from oxidative stress in prenatally GC-exposed offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing-Tong Gao,Jian-Qiang Yuan,Zhi-Yu Zhang,Hou-Ming Zhao,Lu GaoPubMed 36746787
- Disease models & mechanisms 16:2023Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiyun Shen,Shuxin Liu,Xiaojing Wei,Yaping Wang,Lin YangPubMed 36518009
- Cancers 15:2022Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren L Reader,Simon John-McHaffie,Sylvia Zellhuber-McMillan,Tim Jowett,David G Mottershead,Heather E Cunliffe,Elspeth J GoldPubMed 36612143
- Frontiers in pharmacology 13:10197552022FGF10 ameliorates lipopolysaccharide-induced acute lung injury in mice the BMP4-autophagy pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxia Kong,Liling Lu,Daopeng Lin,Lei Chong,Shunhang Wen,Yaokai Shi,Lidan Lin,Liqin Zhou,Hongyu Zhang,Hailin ZhangPubMed 36618911
- International journal of molecular medicine 51:2022Activin A rescues preterm brain injury through a novel Noggin/BMP4/Id2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojuan Su,Junjie Ying,Dongqiong Xiao,Xia Qiu,Shiping Li,Fengyan Zhao,Jun TangPubMed 36524372
- TheScientificWorldJournal 2022:67408532022The Cytokine and Bone Protein Expression by Ellagic Acid-Hydroxyapatite in Bone Remodelling Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDyah Noviana Primasari,Intan Nirwana,Hendrik Setia Budi,Agung Satria Wardhana,Annisa Fitria Sari,Novita Novita,Andika Putri Setyawan,Meircurius Dwi Condro Surboyo,Khairul Anuar ShariffPubMed 36561943
- Development (Cambridge, England) 149:2022Loss of WNT4 in the gubernaculum causes unilateral cryptorchidism and fertility defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbhishek Seth,Juan C Bournat,Olga Medina-Martinez,Armando Rivera,Joshua Moore,Hunter Flores,Jill A Rosenfeld,Liya Hu,Carolina J JorgezPubMed 36448532
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis (PubMed:31363885). Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:25868050, PubMed:8006002). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes (PubMed:25868050, PubMed:29212066). Positively regulates the expression of odontogenic development regulator MSX1 via inducing the IPO7-mediated import of SMAD1 to the nucleus (By similarity). Required for MSX1-mediated mesenchymal molar tooth bud development beyond the bud stage, via promoting Wnt signaling (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity). Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades (PubMed:31363885). For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response (PubMed:31363885).
Alternative names
BMP2B, DVR4, BMP4, DVR4, BMP2B, Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B
Recommended products
Rabbit Polyclonal BMP4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 98 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab39973 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab124715.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-BMP4 antibody (ab39973)
All lanes: Western blot - Anti-BMP4 antibody (ab39973) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
Lane 3: Brain (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Predicted band size: 47 kDa
Observed band size: 42 kDa, 47 kDa
This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomImmunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (ab39973)
ICC/IF image of ab39973 staining primary Rat hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-BMP4 antibody (ab39973)
ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-BMP4 antibody (ab39973) at 1 µg/mL
Lanes 1 and 6: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Mouse Tissue Lysate at 10 µg
Lanes 3 and 8: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lanes 4 and 9: Human heart tissue lysate - total protein (ab29431) at 10 µg
Lanes 10 and 5: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 100 kDa, 47 kDa
This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomWestern blot - Anti-BMP4 antibody (ab39973)
All lanes: Western blot - Anti-BMP4 antibody (ab39973) at 1/1000 dilution
All lanes: mouse brain at 20 µg
SecondaryAll lanes: Dako Cytomation P0260 Conjugation: HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 1min
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