Anti-BMP4 antibody [EPR6211] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (AB271886)

Unpurified ab124715, at 1/100, staining BMP4 in paraffin embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (AB271886)

Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) cells labelling BMP4 with purified ab124715 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (AB271886)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling BMP4 with Purified ab124715 at 1 : 400 dilution (2.44 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (AB271886)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BMP4 with purified ab124715 at 1/120 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

• IP

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Immunoprecipitation - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (AB271886)

ab124715 (purified) at 1 : 60 dilution (2μg) immunoprecipitating BMP4 in A549 whole cell lysate.
Lane 1 (input) : A549 (Human lung carcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab124715 & A549 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab124715 in A549 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

All lanes:

Immunoprecipitation - Anti-BMP4 antibody [EPR6211] (<a href='/en-us/products/primary-antibodies/bmp4-antibody-epr6211-ab124715'>ab124715</a>)

Predicted band size: 47 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-BMP4 antibody [EPR6211] - BSA and Azide free (AB271886)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.