Rabbit Recombinant Monoclonal BMP7 antibody. Carrier free. Suitable for sELISA and reacts with Synthetic peptide - Human, Synthetic peptide - Mouse samples.
Images
![Sandwich ELISA - Anti-BMP7 antibody [EPR23143-116] - BSA and Azide free (Detector) (AB273559), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr23143-116-bsa-and-azide-free-detector-ab273559--sandwich-elisa-img409218.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-BMP7 antibody [EPR23143-116] - BSA and Azide free (Detector) (AB273559)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Synthetic peptide - Human | Tested |
| Synthetic peptide - Mouse | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide - Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis (PubMed:31208997). Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A (PubMed:12667445, PubMed:9748228). Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes (PubMed:12478285). For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, uses also BMPR2 as type II receptor (PubMed:31208997). Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors (PubMed:27923061). Promotes the expression of HAMP, this is repressed by its interaction with ERFE (PubMed:30097509).
Alternative names
OP1, BMP7, Bone morphogenetic protein 7, BMP-7, Osteogenic protein 1, OP-1
Recommended products
Rabbit Recombinant Monoclonal BMP7 antibody. Carrier free. Suitable for sELISA and reacts with Synthetic peptide - Human, Synthetic peptide - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23143-116
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab273559 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human BMP7. The recommended pair for sandwich ELISA is:
Capture: Anti-BMP7 antibody [EPR23143-106] - BSA and Azide free (Capture) ab273557, Human BMP7 Capture Antibody (unconjugated)
Detector: ab273559, Human BMP7 Detector Antibody (unconjugated)
The reference range value is 62.5 - 8000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-BMP7 antibody [EPR23143-116] - BSA and Azide free (Detector) (ab273559), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr23143-116-bsa-and-azide-free-detector-ab273559--sandwich-elisa-img409218.jpg "Sandwich ELISA - Anti-BMP7 antibody [EPR23143-116] - BSA and Azide free (Detector) (ab273559)")
Sandwich ELISA - Anti-BMP7 antibody [EPR23143-116] - BSA and Azide free (Detector) (ab273559)
Sandwich ELISA of Human/Mouse BMP7 Antibody Pair - BSA and Azide free ab253536 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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