AB129156

Anti-BMP7 antibody [EPR5897]

RabMAb
Recombinant
What is this?

(1 Review)

(12 Publications)

Rabbit Recombinant Monoclonal BMP7 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples. Cited in 12 publications.

View Alternative Names

OP1, BMP7, Bone morphogenetic protein 7, BMP-7, Osteogenic protein 1, OP-1

5 Images

Lab

Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (AB129156)

Purified ab129156 at 1/150 immunoprecipitating BMP7 in MCF7 (Human breast adenocarcinoma cell line) whole cell lysate observed at 49 KDa (lanes 1 and 2).

Lane 1 (input) : MCF7 whole cell lysate 10μg

Lane 2 (+) : ab129156 + MCF7 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129156 in MCF7 whole cell lysate

For western blotting, ab129156 at 1/150 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution

All lanes:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 1/15 dilution

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

false

Unknown

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/5000 dilution

Lane 1:

Rat kidney lysate at 20 µg

Lane 2:

Mouse kidney lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

false

Unknown

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

The recombinant peptide should give a band of approximately 13.7 kDa.

All lanes:

Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution

All lanes:

recombinant Human BMP7 (amino acids 316 - 431) at 0.01 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

All lanes:

Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution

Lane 1:

Fetal kidney lysate at 10 µg

Lane 2:

Human prostate lysate at 10 µg

Lane 3:

HT-1376 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 49 kDa

false

Carrier free

Anti-BMP7 antibody [EPR5897] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5897

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/150", "IP-species-notes": "For unpurified use at 1/10 - 1/100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis (PubMed : 31208997). Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A (PubMed : 12667445, PubMed : 9748228). Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes (PubMed : 12478285). For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, uses also BMPR2 as type II receptor (PubMed : 31208997). Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors (PubMed : 27923061). Promotes the expression of HAMP, this is repressed by its interaction with ERFE (PubMed : 30097509).

See full target information BMP7

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Reproductive sciences (Thousand Oaks, Calif.) 32:2970-2984 PubMed40789984

2025

Kuntai Capsules Improve Premature Ovarian Failure by Regulating AMPK-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Ye,Miao Chen,Jiajing Zhong,Haofan Chen,Xinmiao Lin

Heliyon 9:e22487 PubMed38094047

2023

Icariin has the potential to induce the differentiation of bone marrow mesenchymal stem cells into brown fat cells via PDE5A inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Lu,Xiangfeng Xu,Sitong Liu,Zhaozheng Wang,Kai Jiang,Juan Gu,Li Fang,Rong Shen

Heliyon 9:e19934 PubMed37809369

2023

BMP-7 modified exosomes derived from synovial mesenchymal stem cells attenuate osteoarthritis by M2 polarization of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Weixue Sun,Shaozheng Qu,Mingxia Ji,Yanli Sun,Baiqiang Hu

Bioengineered 13:13689-13702 PubMed35707851

2022

Toll-like receptor 9 (TLR9) gene deletion-mediated fracture healing in type II diabetic osteoporosis associates with inhibition of the nuclear factor-kappa B (NF-κB) signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiakai Han,Qian Zheng,Yongxia Cheng,Yong Liu,Yuxin Bai,Bin Yan,Sufen Guo,Jianbo Yu,Xinxin Li,Chong Wang

Journal of nanobiotechnology 19:402 PubMed34863187

2021

Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yitong Guo,Zhuo Wan,Ping Zhao,Mengying Wei,Yunnan Liu,Te Bu,Wenqi Sun,Zhelong Li,Lijun Yuan

Diabetology & metabolic syndrome 13:137 PubMed34801077

2021

CircSMAD4 alleviates high glucose-induced inflammation, extracellular matrix deposition and apoptosis in mouse glomerulus mesangial cells by relieving miR-377-3p-mediated BMP7 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Rina Wu,Zheli Niu,Guangwei Ren,Lin Ruan,Lijun Sun

Molecular therapy : the journal of the American Society of Gene Therapy 30:763-781 PubMed34678513

2021

Bone marrow mesenchymal stem cell-derived exosomal miR-34c-5p ameliorates RIF by inhibiting the core fucosylation of multiple proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Hu,Nan Shen,Anqi Liu,Weidong Wang,Lihua Zhang,Zhigang Sui,Qingzhu Tang,Xiangning Du,Ning Yang,Wantao Ying,Biaojie Qin,Zhitong Li,Lin Li,Nan Wang,Hongli Lin

Science advances 7: PubMed33627425

2021

Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Huayu Zhu,Jun Li,Yize Li,Zhao Zheng,Hao Guan,Hongtao Wang,Ke Tao,Jiaqi Liu,Yunchuan Wang,Wanfu Zhang,Chao Li,Jie Li,Lintao Jia,Wendong Bai,Dahai Hu

eLife 9: PubMed33006314

2020

GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Zhongyu Xie,Jinteng Li,Jiajie Lin,Guan Zheng,Wenjie Liu,Su'an Tang,Shuizhong Cen,Guiwen Ye,Zhaofeng Li,Wenhui Yu,Peng Wang,Yanfeng Wu,Huiyong Shen

FASEB journal : official publication of the Federa 33:3212-3224 PubMed30383450

2018

BMP7 antagonizes proliferative vitreoretinopathy through retinal pigment epithelial fibrosis in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Haipei Yao,Tandi Ge,Yao Zhang,Min Li,Shuai Yang,Hui Li,Fang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BMP7 antibody [EPR5897]

Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (AB129156)

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

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