Rabbit Recombinant Monoclonal BMP7 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples. Cited in 10 publications.
Images
![Western blot - Anti-BMP7 antibody [EPR5897] (AB129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img82916.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)")
![Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (AB129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--immunoprecipitation-img16563.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (AB129156)")
![Western blot - Anti-BMP7 antibody [EPR5897] (AB129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img154594.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)")
![Western blot - Anti-BMP7 antibody [EPR5897] (AB129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img124008.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)")
![Western blot - Anti-BMP7 antibody [EPR5897] (AB129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img28368.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BMP7 antibody [EPR5897] (AB129156)")
Publications
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- Heliyon 9:e224872023Icariin has the potential to induce the differentiation of bone marrow mesenchymal stem cells into brown fat cells via PDE5A inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Lu,Xiangfeng Xu,Sitong Liu,Zhaozheng Wang,Kai Jiang,Juan Gu,Li Fang,Rong ShenPubMed 38094047
- Heliyon 9:e199342023BMP-7 modified exosomes derived from synovial mesenchymal stem cells attenuate osteoarthritis by M2 polarization of macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeixue Sun,Shaozheng Qu,Mingxia Ji,Yanli Sun,Baiqiang HuPubMed 37809369
- Journal of nanobiotechnology 19:4022021Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYitong Guo,Zhuo Wan,Ping Zhao,Mengying Wei,Yunnan Liu,Te Bu,Wenqi Sun,Zhelong Li,Lijun YuanPubMed 34863187
- Diabetology & metabolic syndrome 13:1372021CircSMAD4 alleviates high glucose-induced inflammation, extracellular matrix deposition and apoptosis in mouse glomerulus mesangial cells by relieving miR-377-3p-mediated BMP7 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRina Wu,Zheli Niu,Guangwei Ren,Lin Ruan,Lijun SunPubMed 34801077
- Molecular therapy : the journal of the American Society of Gene Therapy 30:763-7812021Bone marrow mesenchymal stem cell-derived exosomal miR-34c-5p ameliorates RIF by inhibiting the core fucosylation of multiple proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuemei Hu,Nan Shen,Anqi Liu,Weidong Wang,Lihua Zhang,Zhigang Sui,Qingzhu Tang,Xiangning Du,Ning Yang,Wantao Ying,Biaojie Qin,Zhitong Li,Lin Li,Nan Wang,Hongli LinPubMed 34678513
- Science advances 7:2021Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuayu Zhu,Jun Li,Yize Li,Zhao Zheng,Hao Guan,Hongtao Wang,Ke Tao,Jiaqi Liu,Yunchuan Wang,Wanfu Zhang,Chao Li,Jie Li,Lintao Jia,Wendong Bai,Dahai HuPubMed 33627425
- eLife 9:2020GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Li et. al.PubMed 33006314
- FASEB journal : official publication of the Federa 33:3212-32242018BMP7 antagonizes proliferative vitreoretinopathy through retinal pigment epithelial fibrosis in vivo and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaipei Yao et. al.PubMed 30383450
- Stem cells (Dayton, Ohio) 37:270-2832018LncRNA-OG Promotes the Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells Under the Regulation of hnRNPK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu'an Tang,Zhongyu Xie,Peng Wang,Jinteng Li,Shan Wang,Wenjie Liu,Ming Li,Xiaohua Wu,Hongjun Su,Shuizhong Cen,Guiwen Ye,Guan Zheng,Yanfeng Wu,Huiyong ShenPubMed 30372559
- Cellular physiology and biochemistry : internation 46:2601-26152018MiR-301a-3p Suppresses Estrogen Signaling by Directly Inhibiting ESR1 in ERα Positive Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Lettlova et. al.PubMed 29763890
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
| Mouse | Expected | Tested | Not recommended | Not recommended |
| Rat | Expected | Tested | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/150 | Notes For unpurified use at 1/10 - 1/100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis (PubMed:31208997). Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A (PubMed:12667445, PubMed:9748228). Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes (PubMed:12478285). For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, uses also BMPR2 as type II receptor (PubMed:31208997). Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors (PubMed:27923061). Promotes the expression of HAMP, this is repressed by its interaction with ERFE (PubMed:30097509).
Alternative names
OP1, BMP7, Bone morphogenetic protein 7, BMP-7, Osteogenic protein 1, OP-1
Recommended products
Rabbit Recombinant Monoclonal BMP7 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5897
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-BMP7 antibody [EPR5897] (ab129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img82916.jpg "Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)")
Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
Blocking/Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/5000 dilution
Lane 1: Rat kidney lysate at 20 µg
Lane 2: Mouse kidney lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
![Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--immunoprecipitation-img16563.jpg "Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)")
Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)
Purified ab129156 at 1/150 immunoprecipitating BMP7 in MCF7 (Human breast adenocarcinoma cell line) whole cell lysate observed at 49 KDa (lanes 1 and 2).
Lane 1 (input): MCF7 whole cell lysate 10μg
Lane 2 (+): ab129156 + MCF7 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab129156 in MCF7 whole cell lysate
For western blotting, ab129156 at 1/150 dilution and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)
Predicted band size: 49 kDa
![Western blot - Anti-BMP7 antibody [EPR5897] (ab129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img154594.jpg "Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)")
Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
Blocking/Diluting buffer 5% NFDM/TBST
All lanes: Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution
All lanes: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 1/15 dilution
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
![Western blot - Anti-BMP7 antibody [EPR5897] (ab129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img124008.jpg "Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)")
Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
All lanes: Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution
Lane 1: Fetal kidney lysate at 10 µg
Lane 2: Human prostate lysate at 10 µg
Lane 3: HT-1376 lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 49 kDa
![Western blot - Anti-BMP7 antibody [EPR5897] (ab129156), expandable thumbnail](https://content.abcam.com/products/images/bmp7-antibody-epr5897-ab129156--western-blot-img28368.jpg "Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)")
Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
The recombinant peptide should give a band of approximately 13.7 kDa.
All lanes: Western blot - Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution
All lanes: recombinant Human BMP7 (amino acids 316 - 431) at 0.01 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa
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