Rabbit Polyclonal BMP8a/OP-2 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Bone morphogenetic protein 8A aa 300 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Expected |
Mouse | Expected | Tested |
Rat | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (By similarity). Signaling protein involved in regulation of thermogenesis and energy balance. Proposed to increase the peripheral response of brown adipose tissue (BAT) to adrenergic stimulation while acting centrally in the hypothalamus to increase sympathetic output to BAT.Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including spermatogenesis, osteogenesis, steroidogenesis as well as regulation of energy balance (PubMed:22579288, PubMed:31940275). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:31940275). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. In addition, activates the SMAD2/3 pathway (PubMed:31940275).
Bone morphogenetic protein 8A, BMP-8A, BMP8A
Rabbit Polyclonal BMP8a/OP-2 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Bone morphogenetic protein 8A aa 300 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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7.5% SDS PAGE
All lanes: Western blot - Anti-BMP8a/OP-2 antibody (ab154373) at 1/500 dilution
Lane 1: H1299 whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Predicted band size: 45 kDa
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, labeling BMP8a/OP-2 using ab154373 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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